MOULDS, EUSTS, ETC. Ill 



V. Sow some of the spores on moist bread, or in an 

 artificial nutritive solution,^ and before tiie growth of mould 

 is visible to the eye, examine microscopically. Sketch 

 some of the spores in germination, and observe subsequent 

 stages of development. 



VI. Examine, first with a hand lens and afterwards 

 microscopically, zygospores of any of the brown moulds 

 that may be procurable. Study and draw mature speci- 

 mens, and observe as many of the earlier stages of de- 

 velopment as possible. 



Note. — Zygospores are raised artificially with some uncertainty, but 

 beautiful specimens are often found growing spontaneously on decaying 

 mushrooms. 



VII. Write a full description of the black mould as you 

 have studied it, including what you have observed of its 

 habits, length of time required to produce a crop of mould, 

 conditions favorable to its growth, and modes of repro- 

 duction. 



WHEAT RUST. Puccinia graminis, P. 



I. The work is to be conducted with the following 

 different sorts of material : — 



1. Specimens of wheat, gathered a little before harvest, 



infested with rust. 



2. Wheat straw, or stalks of other grasses, gathered at 



harvest time or later in the season, having the black 

 spots containing the winter spores of the same 

 fungus. 



3. Leaves of common barberry with secidium fruits. 



These may be preserved in alcohol in excellent 

 condition for study. 

 II. Beginning with 1, the well-known " rust " of wheat, 

 observe the form and distribution of the spots, sori, their 



1 Directions for making clean cultures are given in the various labora- 

 tory guides. 



