rouL-BROOD. 475 



larva frequently contains as many as one billion of these, 

 spores (28). 



ySS. In the Bulletin Agricole du uepartement de I'Auhs, 

 Mr. Brunei narrates the experiments made by Mr. Marcel 

 Dupont, to breed the bacilli of foul-brood. Knowing that 

 Pasteur used beef-broth in this kind of experiments, Mr. 

 Dupont filled three glass-tubes with unsalted beef-broth, pre- 

 pared according to the directions given by Pasteur, and after 

 sealing and boiling them, to kill any living organisms that 

 might have existed inside, he introduced into two of them, 

 with a fine needle, a small quantity of a liquid, in which 

 -particles from the body of a diseased larva had been dis- 

 solved. One week after, the broth in both of these tubes, was 

 cloudy and full' of bacilli, while the liquid, in the third tube,, 

 had remained clear and unchanged. 



TSO. Description. 



"In most cases the larva is attacked when nearly ready to 

 seal up. It turns slightly yellow, or grayish spots appear on it. 

 It then seems to soften, settles down in the bottom of the cell, 

 in a shapeless mass, at first white, yellow, or grayish in color, 

 soon changing to brown. At this stage it becomes glutinous 

 and ropy; then, after a varjdng length of time, owing to the 

 weather, it dries up into a dark coflfee-oolored mass. Usually 

 the bees make no attempt to clean out the infected cells, and 

 they will sometimes fill them with honey, covering up this dried 

 foul-brood matter at the bottom. 



Sometimes the larva do not die until sealed over. "We have 

 been told that such may be easily detected by a sunken cap- 

 ping perforated by a "pin-hole." This is by no means in- 

 variably the case. Such larvae will often dry up entirely, with- 

 out the cap being perforated or perceptibly sunken, although 

 it usually becomes darker in color than those covering healthy 

 larvae. 



The most fatal misapprehension has been in regard to the 

 smell of the disease. In its first stages there is no perceptible 

 smell, and it is not until the disease has made a considerable 

 progress that any unusual smell would be noticed by most per- 

 sons. In the last stages, when sometimes half or more of the- 



