V.J CONNECTIVE TISSUE. 61 



cesses divide much, but irregularly, and commu- 

 Hicate with the processes of other cells. 

 2. Take a frog, of which the brain and spinal cord 

 have been destroyed^ squeeze the side of the 

 head gently to make the eye bulge out, then 

 slice boldly at the edge of the cornea ; if it is not 

 completely separated, take up the edge with 

 forceps, and cut through the cornea with scissors 

 at its junction with the sclerotic ; in doing this 

 remember that the success of the preparation 

 greatly depends upon the cornea being placed 

 in gold chloride without having been dragged 

 or pulled about; any blood which may be on 

 the cornea should be removed by placing it in 

 a watch-glass containiug normal saline solution 

 and very gently brushing it with a camel-hair 

 brush. Put it in gold chloride "5 p.c. solution 

 for twenty to forty minutes, wash well with 

 water, transfer to water just acidulated with 

 acetic acid, and expose to light". When the 

 cornea has become of a red- or blue-violet colour, 

 put it on a slide with glycerine, and brush or 

 scrape both surfaces to remove the epithelium, 

 then mount in glycerine and examine it under 

 a high power. Where the hexagonal surface 

 epithelium has been removed, there will be seen 

 stained connective-tissue corpuscles, with numer- 

 , ous fine branching processes which anastomose 

 with the similar processes of neighbouring cells. 



1 See Appendix. 



2 Probably it will not be stained until the following day. By the 

 taitaiic acid method (cp. Appendix) the staining ia i^uicker. 



