LESSON VI. 



MODIFICATION OF CONNECTIVE TISSUE 

 AND HYALINE CAETILAGE. 



A. Transition to Fibro-Cartilage. 



1. Take a piece of intervertebral cartilage', which 

 has been treated with chromic or picric acid and 

 subsequently with alcohol, and imbed it in the 

 following manner. At one end of a small oblong 

 block of paraffin B' scoop out a small hole ; with 

 blotting-paper remove the excess of alcohol from 

 the piece of cartilage and place it in the hole so 

 that the plane of the uppermost surface is at 

 right angles with the plane of the vertebral face. 

 Pour over it a little of the paraffin mixture heated 

 so as to be just melted ; remove any bubbles that 

 may be present and keep the tissue in its proper 

 position by means of a heated needle ; when the 

 whole is thoroughly solid pare down the paraffin 



1 Cut off from one face of a vertebra of a rabbit a thin slice about 

 1 mm. thick, leaving the intervertebral cartilage in connection with it ; 

 divide the more or less circular piece so obtained into four parts, and 

 treat with chromic or picric acid to dissolve the salts (cp. Appendix). 



2 Cp. Appendix. 



