VI.] MODIFICATION OF CONNECTIVE TISSUE, fiTO. 6& 



to the surface of the cartilage, at the same time 

 bevelling the edges. In cutting the sections, 

 cover the tissue and the blade of the razor with 

 spirit (for convenience from a wash-bottle) and 

 remove the sections by means of a camel-hair 

 brush from the razor to a watch-glass. With 

 the platinum "lifter" transfer them to hsema- 

 toxylin and treat as in § 7, Lesson iv. 

 It is to be remembered that if the chromic acid* 

 has not been completely removed from the tissue 

 the staining will be very imperfect ; in this case 

 the sections should be placed in a little NajCO, 

 1 p.c. for a few minutes and then washed with 

 water before staining. Observe in the sections 



a. The softened bone passing into 



b. The thin layer of hyaline cartilage ; outside 

 this 



c. The arrangement of the cartilage cells in 

 . rows, and the concomitant appearance of 



fibres in the matrix, this gradually but 

 rapidly merging into 



d. Fibro-cartilage consisting of bundles of white 

 fibrous tissue and of rather large cells arranged 

 in rows between the fibrous bands; these cells 

 are like the cells of hyaline cartilage and 

 each has a thin hyaline capsule around it.^ 



e. Towards the outer part of the cartilage, the 

 capsules and cells become smaller and more 



1 Mullet's fluid, potassium bichromate and ammonium bichromate, 

 must similarly be completely removed from tissues which are to be 

 stained. 



