102 ELEMENTARY PHTSIOLOGT. [iX. 



frequently shaking. After 3 or 4 hours most of 

 the myosin of the muscle will have been con- 

 verted into syntonin or acid-albumin, and dis- 

 solved. Syntonin may be prepared in a similar 

 way from white of egg or from serum. 

 Filter and carefully neutralize the filtrate with a 

 weak solution of sodium carbonate (1 c.c. of HCl 

 "1 p.c. requires about 1'5 c.c. of Na^ 00, "1 p.c. to 

 neutralize it), a copious bulky precipitate of 

 syntonin takes place ; if too much alkaline salt 

 is added the syntonin will be converted into 

 alkali albuminate and redissolved (cp. § 16). 

 rUter and wash the precipitate once on the filter 

 with water, then break through the filter paper 

 and wash the precipitate into a beaker with 

 water. The syntonin is thus obtained suspended 

 in water. Place 2 c.c. in each of 2 test-tubes. 



a. Add a little HCl "1 p.c, the precipitate at 

 once dissolves and is not . precipitated on 

 boiling; cool under a tap, and test for pro- 

 teids e.g. with acetic acid and potassium 

 ferrocyanide (cp. Less. iii. § 16). 



b. Boil, cool under a tap, and add a little HCl 

 •1 p.c; the precipitate suspended in the water 

 has been coagulated by boiUng and is no 

 longer soluble in dilute acids. 



16. Alkali Albuminate. 



Treat a little washed muscle as in § 15 but with 

 NaHO "1 p.c. instead of with HCl. On neutral- 

 izing a precipitate of alkali albuminate will be 



