XIX.] STRUCTURE OF LIVER. GLYCOGEN, 209 



■wine colour will result which will rapidly 

 disappear if much glycogen is present, if so 

 add more iodine until the colour is permanent, 

 Waim gently ; the colour will disappear, but 

 will return on cooling (unless inuch pA)teid 

 matter be present). 



b. Test 5 c.c, for sugar with -Trom^er's test 

 (cp. Lesson xv. D § 7); a small quantity 

 only will be found, 



c. Add to 10 c.c. (neutralizing if acid) a little 

 saliva, or artificial pancreatic juice, and place 

 it in the warm chamber; the opalescence will 

 disappear, and the fluid become transparent, 



a. To 5 c.c. of this add iodine as before; the 

 port- wine colour will not appear, showing 

 that glycogen is no longer present. 



/S. Test the other 5 c.c, for sugar; much 

 more than before will be found. 



3. After some hours' warming, make a decoction of 

 the other half of the liver. It ■will probably 

 be acid, neutralize ■with sodium carbonate and 

 filter. 



a. The decoction will be clearer, more trans- 

 parent, and less milky, 



b. It ■will give less port-wine colour with iodine. 



c. It ■will contain sugar in abundance, 



L. 14 



