256 GENERAL THERAPEUTICS FDR VETERINARIANS 



nodular swellings of the lymph glands up to the size of a hazel- 

 nut may be felt; later, these rupture; on post-mortem examination, 

 purulent lymph glands and glanders abscesses are found, the latter 

 especially in the testicles (glandular orchitis); also glandular 

 nodules in the spleen, liver and limgs. A negative result from the 

 inoculation does not exclude the presence of glanders. When the 

 result is positive, a diagnosis of glanders is only justified when the 

 bacillus mallei is also demonstrated in cultm-es (pseudo-glanders!). 

 Other inoculation animals are the horse and the ass, the latter 

 contracting acute glanders and usually dying in about 8 days; 

 also cats, which, after subcutaneous (dorsal) inoculation, present 

 symptoms similar to those exhibited by guinea-pigs. Concerning 

 injection with mallein, see page 260. 



4. TuBEKCULOsis. — The best inoculation animal is the guinea- 

 pig. After the usual intraperitoneal inoculation (milk), numerous 

 fresh tubercles of the size of millet seed appear in about three 

 weeks upon the peritoneum and id the spleen, liver, and lymph 

 glands. Following subcutaneous injection (mucus from the 

 lungs), a purulent ulcer develops at the point of injection and the 

 neighboring lymph glands become swollen; after 3 to 4 weeks 

 numerous tubercles form in the internal organs. After intra- 

 muscular inoculation (rapid inoculation), the neighboring lymph 

 glands are transformed in ten days into firm nodules of the size 

 of small peas, which may be extirpated and examined microscopic- 

 ally. Intramammary inoculation and subcutaneous injection with 

 simultaneous crushing of the lymph glands are followed in five 

 days by the appearance of nodular swellings in the glands con- 

 cerned. Test inoculations of guinea-pigs is the most certain method 

 of clinically diagnosing tuberculosis. Concerning inoculation with 

 tuberculin, see page 258. ' 



Fowl Choleea. — ^A pigeon is inoculated subcutaneously with 

 blood from a dead bird. A drop of blood from the dead bird is 

 introduced under the skin of the breast of the pigeon by means of 

 a lancet to the depth of a millimetre. If cholera is present, the 

 musculature beneath the point of inoculation becomes necrotic 



