CONTENTS. 



INTRODUCTION. 



PAUK8 



The overthrow of the doctrine qf spontaneous generation 

 and the further application of the law : "Omne vivum ex 

 vivo" — The birth of modern bacteriology . . . 13-26 



CHAPTER I. 



Definition of bacteria — Their place in nature — Differ- 

 ence between parasites and saprophytes — Nutrition of 

 bacteria — Products of bacteria — Their relation to oxygen 

 — Influence of temperature upon their growth . . 27-35 



CHAPTER II. 



Morphology of bacteria — Grouping — Mode of multi- 

 plication — Spore-formation — Motility .... 36-46 



CHAPTER III. 



Principles of sterilization by heat — Methods employed 

 — Discontinued sterilization — Sterilization under pressure 

 — Apparatus employed — Chemical disinfection and steril- 

 ization .... . ... 47-67 



CHAPTER IV. 



The principles involved in the methods of isolating bac- 

 teria in pure cultures by the plate method of Koch — 

 Materials employed . .... 68-72 



CHAPTER V. 



Preparation of nutrient media — Bouillon, gelatin, agar- 

 agar, potato, blood-serum, etc. 73-96 



