viii • CONTENTS. 



CHAPTEE VI. 



PAGES 



Preparation of tubes, flasks, etc., in which media are to 

 be preserved 97-100 



CHAPTEE VII. 



Technique of making plates — Esmarch tubes, Petri 

 plates, etc 101-110 



CHAPTEE VIII. 



The incubating oven — Gas pressure regulator — Thermo- 

 regulator — Safety burner for heating the incubator . 111-118 



CHAPTEE IX. 



The study of colonies — Tlieir naked-eye appearances 

 and their variations under different conditions — Difler- 

 ences in the structure of colonies of different species of 

 bacteria— Stab cultures — Slant cultures .... 119-123 



CHAPTEE X. 



Methods of staining — Solutions employed — Preparation 

 and staining of cover-slips — Preservation and preparation 

 of tissues for section cutting — Staining of tissues, general 

 and special methods ........ 124-158 



CHAPTEE XI. 



Systematic study of an organism — Points to be con- 

 sidered in identifying an organism as a definite species . 159-184 



CHAPTEE XII. 



Inoculation of animals — ^The various methods : subcu- 

 taneous ; intra-venous ; into the great serous cavities ; into 

 the anterior chamber of the eye — Observation of animals 

 after inoculation 185-203 



CHAPTEE XIII. 



Post-mortem examination of animals — Bacteriological 

 examination of tissues at autopsy — Disposal of tissues and 

 disinfection of instruments after the examination . . 204-208 



