B. ANTHRACIS: EXPERIMENTS. 



to be explained ? Do the cultures which were made 

 from these flasks at the time of their removal from the 

 incubators act in the same way toward animals as the 

 organisms growing in the flasks ? Is the action of each 

 of these cultures the same for mice, guinea-pigs, and 

 rabbits ? 



Prepare a 2 per cent, solution of sulphuric acid in 

 distilled water; suspend in this a number of anthrax 

 spores ; at the end of three, six, and nine days at 35° C. 

 inoculate both a guinea-pig and a rabbit. Prepare cul- 

 tures from this suspension on the third, sixth, and ninth 

 days ; when the cultures have developed inoculate a rab- 

 bit and a guinea-pig from the culture made on the ninth 

 day. Should the animal survive, inoculate it again 

 after three or four days with a culture made on the 

 sixth day. Do the results appear in any way peculiar ? 



