LABORATORY AND TEACHING METHODS 



637 



8. Vitality on Culture Media. — BrieJ, moderate, long. 



9. Temperature Relations. — Thermal death point (ten minutes exposure in 

 nutrient broth when this is adapted to growth of organism) °C. 



10. Killed readily by drying, resistant to drying. 



11. Per cent, killed by freezing (salt and crushed ice or liquid air). 



12. Sunlight. — Exposure on ice in thinly sown agar plates; one-half plate cov- 

 ered (time fifteen minutes), sensitive, non-sensitive. 



Per cent, killed 



13. Acids produced 



14. Alkalis produced 



15. Alcohols 



16. Ferments. — Pepsin, trypsin, diastase, invertase, pectase, cytase, tyrosinase, 

 oxidase, peroxidase, lipase, catalase, glucase, galactose, lab, etc. 



1 7. Crystals formed 



18. Effects of Germicides 



Substance 



Method used 



I 5 



.t; 3 

 3i 



IV. PATHOGENICITY. 



1. Pathogenic to Animals. — Insects, crustaceans, fishes, reptiles, birds, mice, 

 rats, guinea pigs, rabbits, dogs, cats, sheep, goats, cattle, horses, monkeys, man. 



2. Pathogenic to Plants. — 



Toxins. — Soluble, endotoxins. 



NON-TOXIN forming 



Immunity (bactericidal) 



Immunity (non-bactericidal) 



Loss OF Virulence on Culture Media. — Prompt, gradual, not observed 

 in months. 



