APPENDICES III, IV, V 693 



C. Use of Hydrostatic Pressure. — Boucherie method with sulphate of copper. 



D. Use of A ir Pressure (Open-tank treatment) . 



E. Use of Steam Pressure. — The liquids commonly used are chloride of zinc, 

 coal-tar creosote, mixture of chloride of zinc and of creosote, gases of tar 

 oils (thermo-carbolization), heavy petroleums. 



Preservation of Wood by Air Drying or Kiln Drying. Bibliography. — Schenck, 

 C. A.: Logging, Lumbering or Forest Utilization, 1913, and the following bulletins: 

 Bureau of Forestry and late Forest Service, U. S. Dept. Agr.: No. 41, Seasoning of 

 Timber; No. 50, Cross Tie Forms, Etc. with Reference to Treated Timbers; No. 51, 

 Condition of Treated Timbers Laid in Texas, February, 1902; No. 78, Wood Preser- 

 vation in the United States; No. 84, Preservative Treatment of Poles; No. 107, 

 Preservation of Mine Timbers; No. 118, Prolonging Life of Crossties; No. 126, 

 Preservative Treatment of Red Oak and Hard Maple Cross Ties, etc. 



APPENDIX IV 



CULTURE OF MUSHROOMS 



Tissue Culture of Fleshy Fungi. — Consult Duggar, B. M.: The Principles of 

 Mushroom Growing and Mushroom Spawn Making. Bull. No. 85, Bureau of Plant 

 Industry, 1905: 18. 



This method is applicable to the mushroom and to 68 other species of fleshy 

 fungi listed by Duggar. 



A young sporophore of Agaricus campestris is taken and broken open longitudi- 

 nally. A number of pieces are carefully removed with a sterile scalpel to a sterile 

 Petri dish on a number of nutrient media such as bean pods, manure and leaf mould. 

 From this and numerous other similar tests it was ascertained that when the mush- 

 rooms, from which the pieces of tissue are taken, are young and healthy, there is 

 seldom an instance in which growth does not result. It was easily shown that failure 

 to grow was generally due to advanced age of the mushroom used, to an unfavorable 

 medium, or to bacterial contamination. 



APPENDIX V 



SYNOPSIS or THE FAMILIES AND PRINCIPAL GENERA OF THE MYXOGASTRALES 



Suborder I. Exosporeae. — Spores developed outside of the sporophore. 



Family I. Ceratiomyxace^. — Sporophores membranous, branched; spores 

 white, borne singly on filiform stalks arising from the areolated 

 sporophore. 

 Suborder II. Endosporea;. — Spores developed inside the sporangium, aethalium or 

 plasmodiocarp. 

 A. Spores violet-brown, or ptirplish gray (ferruginous in Stcmonilis frrniginca 

 and 5. flavogenila, colorless in Echinostelium). 

 la) Sporangium provided with lime (Calcium carbonate). 



