APPENDIX. 



LIST OF KEAGENTS. 



SALT SOLUTION, -75 per cent. 



Prepa/raUon. — Dissolve 7"5 grms. common salt in 1 litre water. 

 Use. — Living or fresh tissues are usually examiaed in it. 



GLTCEBIKE. — Used either pure, or diluted with an equal bulk of 

 water. 



Use. — For mounting microscopical objects. 



ACETIC ACID, 1 per cent, solution. 



Prepa/raUon. — Dissolve 10 c.c. glacial acetic aeid in water : 



make up to 1 litre with water. 

 Use. — For clearing tissues, and especially for rendering nuclei 



more distinct. 



OSUIC ACID, 2 per cent, solution. 



Preparation. — Dissolve 1 grm. osmic acid crystals in 50 c.c. 



distilled water. It must be kept in the dark. 

 Use. — For kiUing microscopic animals, and for staining fets. 



Before use it is to be diluted with 1 to 3 times its bulk of 



water. 



CHBOMIC ACID, 10 per cent, solution. 



Preparation. — Dissolve 10 grms. chromic acid crystals in 



100 c.c. water. 

 Use. — With or without addition of nitric acid for decalcifying 

 bone, etc. As a hardening reagent it must be reduced to 

 a 1 per cent, or '5 per cent, solution by addition of water 

 before use. 



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