418 APPENDIX 



MtfLLER'S TLTJID. 



Preparation. — Dissolve 25 grms. potassium bichromate and 



10 gnns. sodiimi sulphate in 1 litre water. 

 Use. — Hardening reagent. It also dissolves intercellular sub- 

 stance, so that the cells may be separated by teasing. 



COBBOSIVE SUBIimATE, solution. 



Prepa/ration. — Saturate water with mercuric chloride : 1 litre 



dissolves about 70 grms. 

 Use. — (1) As a hardening reagent it is used cold. 



(2) For killing small animals it may be used hot or cold. 



FICBIC ACID, saturated solution. 



Prepa/ration. — Saturate water with picric acid, by shaking it 

 up ■with the crystals. Allow to stand : decant before use. 

 Use. — Hardening reagent. 



FICBIC ACIS, Kleinenberg's solution. 



Preparation. — To 250 c.c. saturated solution of picric acid add 

 5 c.c. strong sulphuric acid : filter : make up to 1 litre with 

 water. 



Use. — Hardening and decalcifying reagent. 



ABSOLTTTE ALCOHOL. 



Use. — Hardening, reagent, but most used for dehydrating a 

 specimen before putting it into oil of cloves or turpentine. 



ALCOHOL (Spirit). 



It is used of the following strengths : 90 per cent., 70 per cent., 

 50 per cent., 30 per cent. 



Prepa/ration. — 90 per cent, alcohol is the ordinary ' methylated 

 spirit.' The weaker solutions are obtained by diluting it 

 with water. The specific gravity of each is as follows : 

 90 per cent. -833 ; 70 per cent. •889 ; 50 per cent. -933 ; 

 30 per cent. •965. 



Use. — (1) For preserving specimens ; not weaker than 70 per 

 cent. 



(2) Hardening reagent ; 90 per cent. 



(3) For dissolving the intercellular substance of certain 



tissues, so that the cells may be isolated by 

 teasing ; 30 per cent. 



