420 APPENDIX 



(b) Saturate 70 per cent, alcohol with aliim. 



Mix 100 C.C. solution (a) with 800 c.c. solution (b) : 

 allow to stand a week. Decant and add a few drops 

 of saturated solution of hsematoxylin in absolute 

 alcphol. 



The solution should be of a deep violet-blue colour. 



If it be reddish, add a solution of sodium carbonate 



drop by drop tiU the -violet-blue colour is obtained. 



Use. — Stain. The specimen must be previously placed in 



strong alcohol. It wiU not give satisfactory results with 



tissues which contain acid. 



OIL OF CLOVES. 



Ihe.- — Clearing reagent, i.e. it is used to remove alcohol from 

 an object before mounting it in Canada balsam. 



TURPENTINE. 



Use. — Clearing reagent ; bat chiefly used for dissolving parafBn 

 from sections before mounting them. 



CBEASOTE AND TUSFENTINE. 



Prepa/ration. — Mix 100 c.c. creasote and 400 c.c. turpentine. 



Allow to stand tiU clear. 

 J7s6.— Same as turpentine, but must not be used for sections 



cemented down vrith shellac. 



CANADA BALSAU. 



Preparation. — May be used as bought from the chemist, or 

 diluted to a suitable consistency with benzol or turpentine. 

 Still better, evaporate to dryness ; powder, and dissolve 

 in chloroform, benzol, or turpentine. 



Use. — Mounting medium. Before being mounted in it, the 

 specimen must be dehydrated with absolute alcohol, and 

 cleared with oil of cloves or otier clearing reagent. 



CASKINE INJECTION ELTTID. 



Prepa/ration. — Eub up carmine in water, add ammonia drop 

 by drop, till the carmine is dissolved. Filter, evaporate 

 at a gentle heat, stirring constantly to get rid of the am- 

 monia. Bub up the residue in camphor-water. 



Use. — For injecting small animals or portions of animals. It 

 must be well shaken before use. 



