288 



BLOOD AND BLOOD EXAMINATION 



that the line of CO-Hb does not disappear upon addition of reduction agents, 

 for example, ammonium sulphate, because CO is firmly combined with hemo- 

 globin; while the spectrum of 0-Hb is changed by the same agent to that of 

 reduced Jiemoglolin. 



A a B C D E b 



'lO 60 60 70 80 00 



ill Ml ,1,1 M,l,,.,lMM|ii.ilrMllMillinilliiiliiii1 mill 



Fio. 16. — The Various Absorption Spectra of Hemoglobin. In all of the spectra the 

 various Fraunhofer lines and a scale in millimeters are drawn. (After Landois.) 



Finally, the slightest degree of hemoglohinemia may be demonstrated with 

 certainty by the spectroscope in those cases in which ocular examination 

 leaves a doubt as to whether or not the serum contains hemoglobin. 



VIII. AGGLUTINATIVE REACTIONS 



To the law of immunity which has made us acquainted with the vital 

 importance of functional changes in the blood we owe a valuable diagnostic 

 method. We know that in the natural infections of man, as well as in 

 animal experiment, peculiar changes occur in the blood serum, under the 



