39 



The average of these 5 clusters gives the following result : — 



Dead eggs, . . . 19 = 8.30 per cent., or about 8 per cent. 



Eggs apparently alive, 210 =91.70 per cent., or about 92 per cent. 



Total, .229 eggs. 



Since a normal cluster contains, on the average, 433 eggs with ap- 

 parently living embryos, and this locality in which Flacherie had pre- 

 vailed contained only 210 eggs with apparently living embryos on the 

 average per cluster, 2 clusters were necessary to equal a normal one. 

 The number of fresh clusters, which were estimated to be about 3,500, 

 must therefore be reduced to about 1,750. The number of apparently 

 living eggs had decreased in this locality, after the wilt had operated, 

 to about 14.5 per cent. 



Marsh-field, Mass, 



Division Agent, John A. Fabley; Local Superintendent, P. R. Livermokb. 



I did not visit this locality, but the experiment was undertaken by 

 the local superintendent with the assistance of Mr. Joseph Shermaun 

 of Marshfleld, who raised the caterpillars according to my instructions. 

 On visiting Marshfleld on June 24, 1910, it was found that Mr. Sher- 

 mann's caterpillars, most of which were ready to undergo the fourth 

 molt, were sick with Flacherie. He was instructed to expose the ma- 

 terial, with the assistance of the local superintendent, in a forest in- 

 fested with the gypsy moth. This was done on June 26, 1910, in a 

 forest of 10 acres. On Oct. 25, 1910, the local superintendent sent, 

 upon my request, a number of fresh egg clusters from the locality 

 where the disease had been planted, and he remarked that the number 

 of egg clusters were considerably decreased in comparison with those 

 present in the spring of 1910. Unfortunately, further detailed com- 

 munications regarding this locality were not received. The first 5 

 clusters, which were taken from the top of the shipment, were exam- 

 ined microscopically. The result was as follows : — • 



Cluster 1. 

 Unfertilized eggs, ... 1 



„ .,, , , ,_ in f = 4.26 per cent., or about 4 per cent. 



Eggs with dead embryos, . 10 J 



Eggs apparently alive, . . 247 =95.74 per cent., or about 96 per cent. 



Total, .... 258 eggs. 



Cluster 2. 

 Unfertilized eggs, ... 8 



, , , , ._ ,— 13.47 per cent., or about 13.5 per cent. 



Eggs with dead embryos, . 65 J 



Eggs apparently alive, . 469 =86.53 per cent., or about 86.5 per cent. 



Total, . . .542 eggs. 



