282 



CLEAN MILK 



growth axis spiral spirtllace^; 



cell flexile Spirochaeta 



cell not flexile 

 flagellate 



three or less, polar Microspira 



bundle, polar Spirillum 



not flagellate Spirosoma 



growth axis not spiral BACTERIACE./E 



flagellate 



peritrichic Bacillus 



polar Pseudomonas 



not flagellate Bacterium 



growth axis not ^> transverse axis coccaceve 



flagellate 



cell-div. in 2 planes Planococcus 



cell-div. in 3 planes Planosarcina 



not flagellate 



cell-div. in 1 plane Streptococcus 



cell-div. in 2 planes Micrococcus 



cell-div. in 3 planes Sarcina 



5 Species 



20 

 5 



=75 



47 



216 



3 



3 



36 

 14 



SCHEME FOR EXAMINATION OF PURE BACTERIAL CULTURES,. 

 FOR IDENTIFICATION OF SPECIES 



((Adopted by the Society of American Bacteriologists, Dec. 31, '07 : Committee 

 on Identification of Species, Chester, Gorham and E. T. Smith. ) 



I. MORPHOLOGY. 



1. Vegetative Cells. — Medium used; temperature ; age in days. Form, round, 



short rods, long rods, short chains, long chains, filaments, commas, short 

 spirals, long spirals, Clostridium, cuneate, clavate, curved. Limits of size. 

 Size of majority. Ends, rounded, truncate, concave. 

 Agar Hanging-Block. — Orientation (grouping). Chains (No. of elements). 

 Short chains, long chains. Orientation of Chains, parallel, irregular. 



2. Sporangia. — Medium used; temperature; age in days. Form, elliptical, short 



rods, spindled, clavate, drumsticks. Limits of Size. Size of Majority. 

 Agar Hanging-Block. — Orientation (grouping). Chains (No. of elements). 



Orientation of Chains, parallel, irregular. 

 Location of Endospores, central, polar. 



3. Endospores. — Form, round, elliptical, elongated. Limits of Size. Size of 



Majority. Wall, thick, thin. Sporangium wall, adherent, not adherent- 

 Germination, equatorial, oblique, polar, bipolar, by stretching. 



4. Flagella. — No.; Attachment, polar, bipolar, peritrichiate. How Stained. 



5. Capsules. — Present on. 



6. Zoogloea. — Pseudozoogloea. 



7. Involution Forms. — On; in days at °C. 



