CLASSIFICATION OF BACTERIA 283 



8. Staining Reactions. — 1 :I0 watery fuchsin, gentian violet, carbol fuchsin, 

 Loeffler's alkaline methylene blue. Special Stains: Gram; Glycogen; 

 Fat; Acid fast; Neisser. 



II. CULTURAL FEATURES. 



1. Agar Stroke. — Growth, invisible, scanty, moderate, abundant. Form of 



growth, filiform, echinulate, beaded, spreading, plumose, arborescent, 

 rhizoid. Elevation of growth, flat, effuse, raised, convex. Lustre, glisten- 

 ing, dull, cretaceous. Topography, smooth, contoured, rugose, vcrrucose. 

 Optical Characters, opaque, translucent, opalescent, iridescent. Chromo- 



genesis. Odor, absent, decided, resembling Consistency, slimy, 



butyrous, viscid, membranous, coriaceous, brittle. Medium, grayed, 

 browned, reddened, blued, greened. 



2. Potato. — Growth, scanty, moderate, abundant, transient, persistent. Form 



of growth, filiform, echinulate, beaded, spreading, plumose, arborescent, 

 rhizoid. Elevation of growth, flat, effuse, raised, convex. Lustre, glisten- 

 ing, dull, cretaceous. Topography, smooth, contoured, rugose, vcrrucose, 

 Chromogenesis. Pigment in water, insoluble, soluble; other solvents. 

 Odor, absent, decided, resembling. . . Consistency, slimy, butyrous, viscid, 

 membranous, coriaceous, brittle. Medium, grayed, browned, reddened, 

 blued, greened. 

 Z. Loeffler's Blood Serum. — Stroke, invisible, scanty, moderate, abundant. 

 Form of growth, -filiform, echinulate, beaded, spreading, plumose, arbores- 

 cent, rhizoid. Elevation of growth, flat, effuse, raised, convex. Lustre, 

 glistening, dull, cretaceous. Topography, smooth, contoured, rugose, 

 verrucose. Chromogenesis. Medium, grayed, browned, reddened, blued, 

 greened. Liquefaction begins in....d, complete in....d. 



4. Agar Stab. — Growth, uniform, best at t-op, best at bottom; surface growth 



scanty, abundant; restricted, wide-spread. Line of puncture, filiform, 

 beaded, papillate, villous, plumose, arborescent; liquefaction. 



5. Gelatin Stab. — Growth, uniform, best at top, best at bottom. Line of 



puncture, filiform, beaded, papillate, villous, plumose, arborescent. Lique- 

 faction, crateriform, napiform, infundibuliform, saccate, stratiform; begins 



in d, complete in d. Medium, fluorescent, broivncd. 



5. Nutrient Broth. — Surface growth, ring, pellicle, flocculent, membranous, 

 none. Clouding, slight, moderate, strong; transient, persistent; none; 

 fluid turbid. Odor, absent, decided, resembling. ■ . Sediment, compact, 

 flocculent, granular, flaky, viscid on agitation, abundant, scant. 



7. Milk. — Clearing without coagulation. Coagulation, prompt, delayed, absent. 



Extrusion of whey begins in days. Coagulum, slowly peptonized, 



rapidly peptonized. Peptonization begins on d, complete on d. 



Reaction, id, 2d, 4d, iod, 20d. Consistency, slimy, viscid, unchanged. 

 Medium, browned, reddened , blued, greened. Lab ferment, present, absent. 



8. Litmus Milk. — Acid, alkaline, acid then alkaline, no change. Prompt re- 



duction, no reduction, partial slow reduction. 



9. Gelatin Colonies. — Growth sloiv, rapid. Form, punctiform, round, irregu- 



lar, ameboid, mycelioid, filamentous, rhizoid. Elevation, flat, effuse, raised, 

 convex, pulvinate, crateriform (liquefying). Edge, entire, undulate, lobate, 

 erase, lacerate, fimbriate, filamentous, floccose, curled. Liquefaction, cup> 

 saucer, spreading. 



