284 CLEAN MILK 



10. Agar Colonies. — Growth, slow, rapid (temperature). Form, punctiform, 

 round, irregular, ameboid, myceloid, filamentous, rhizoid. Surface smooth, 

 rough, concentrically ringed, radiate, striate. Elevation, flat, effuse, raised, 

 convex, pulvinate, umbonate. Edge, entire, undulate, lobate, erose, lacerate, 

 fimbriate, floccose, curled. Internal structure, amorphous, finely-, coarsely 

 „ granular, grumose, filamentous, floccose, curled. 



"11. Starch Jelly. — Growth, scanty, copious. Diastasic action, absent, feeble, 

 profound. Medium stained'. 



12. Silicate Jelly (Fermi's Solution). — Growth, copious, scanty, absent. 



Medium stained. 



13. Cohn's Solution. — Growth, copious, scanty, absent. Medium, fluorescent, 



non-fluorescent. 



14. Uschinsky's Solution. — Growth, copious, scanty, absent. Fluid, viscid, 



not viscid. 



15. Sodium Chloride in Bouillon. — Per cent inhibiting growth. 



16. Growth in Bouillon over Chloroform, unrestrained, feeble, absent. 



17. Nitrogen. — Obtained from peptone, asparagin, glycocoll, urea, ammonia 



salts, nitrogen. 



18. Best media for long-continued growth. 



19. Quick tests for differential purposes. 



III. PHYSICAL AND BIOCHEMICAL FEATURES. 



1. Gas Production from Sugar. — (a) Amount in percentage of tube length 



{closed arm of fermentation tube), (b) H/CO2 formula, (c) Acidity 

 by titration, dextrose, saccharose, lactose, maltose, glycerin, mannit. 



2. Ammonia production, feeble, moderate, strong, absent, masked by acids. 



3. Nitrates in nitrate broth. — Reduced, not reduced. Presence of nitrites; 



ammonia ; presence of nitrates ; free nitrogen. 



4. Indol production, feeble, moderate, strong. 



5. Toleration of Acids: great, medium, slight. Acids tested. 



6. Toleration of Na O H: great, medium, slight. 



7. Optimum reaction for growth in bouillon, stated in terms of Fuller's 



scale. 



8. Vitality on culture media: brief, moderate, long. 



9. Temperature relations. — Thermal death point (10 minutes exposure in 



nutrient broth when this is adapted to growth of organism) C. 



Optimum temperature for growth C. ; or best growth at 15° C, 



20 C, 25° C, 30° C, 37° C, 40 °C, 50 C, 6o° C. Maximum temperature 

 for growth C. Minimum temperature for growth C. 



10. Killed readily by drying: resistant to drying. 



11. Per cent, killed by freezing (salt and crushed ice or liquid air). 



12. Sunlight: Exposure on ice in thinly sown agar plates: one-half plate 



covered (time 15 minutes), sensitive, not sensitive. Per cent killed. 



13. Acids produced. 



14. Alkalies produced. 



15. Alcohols. 



16. Ferments; pepsin, trypsin, diastase, invertase, pectase, cytase, tyrosinase, 



oxidase, peroxidase, lipase, catalase, glucase, galactose, lab, etc. 



17. Crystals formed. 



18. Effect of germicides. 



