Zool— Vol. I.] EISEN—PLASMOCYTES. 23 



of a narrow crescent, as in figs, lib and 32^ ,• when 

 larger it becomes conelike, and as it recedes from the nu- 

 cleus it assumes a spherical form (fig. 25a, etc.). As the 

 plasmocytoblast grows and tends to separate from the nu- 

 cleus, it carries with it either the whole of the granosphere, 

 as in figs. 250 and 38$, or it leaves behind a narrow crescent 

 of granosphere close to the nucleus, as, for instance, seen in 

 figs. 32^, 39, etc. The part that moves away always con- 

 tains the centrosomes or inner spheres, while the part that is 

 left behind appears homogenous throughout, without trace 

 of a microcentrum, except in case the microcentrum has 

 divided, when a later emigration of a plasmocyte may take 

 place. 



While the granosphere generally stains much darker, 

 violet dark in contrast to other spheres which stain blue, 

 this is not always the case, and for some reason or other the 

 staining is inverted even on the same slide. In figs. 27 and 

 33 the tint it has taken is deep blue and the only differen- 

 tiation is in regard to the intensity of the stain. Figs. 21, 

 24, 29, 32, 34, and 35 contain normally stained grano- 

 spheres, while figs. 27, 31, and 33 show a granosphere in 

 which we find no trace of the red. This is to a great ex- 

 tent due to the time allowed the toluidine to stain. When 

 too long the differentiation becomes less apparent, as the 

 blue will quickly drive away the pink and violet. An im- 

 mersion in the toluidine for three minutes will generally give 

 the best differentiation, and even five minutes exposure is 

 generally sufficient to destroy the differential effects. 



The granosphere is also distinguished by its granulated 

 protoplasm which is always quite prominent. This granu- 

 lation is not even, but irregular, both as regards the size of 

 the granules and their distribution. The periphery of the 

 granosphere is nearly always rather even, pressing as it 

 does against the hyalosphere. The contrast between these 

 two spheres is sharp and striking, as in fig. 21. In size 

 the granosphere is variable. Frequently it is very large, as 

 in figs. 2i«, 22, 35, etc.; at other times it is much smaller, 

 like a thin crescent, as in fig. 23. It frequently happens 



