Zool.— Vol. I.] RISEN— PLASMOCYTES. II 



toluidine blue. I wish especially to call attention to the star- 

 like pink-colored zone in the center, which appears to me 

 identical with the granosphere. It is brought out only after 

 several hours immersion in toluidine. The centrosomes are 

 rarely stained by the toluidine, and the archoplasmic spheres 

 are much less distinct than when stained with Ehrlich-Biondi. 

 This granosphere in the leucocytes is exceedingly delicate 

 but nevertheless distinct. I have never seen any rays reach 

 to the cell wall; they always stop in the cytoplasm. Rays 

 are frequently seen in the leucocytes extending from the 

 archoplasmic region toward the periphery of the cell, but 

 they consist of two distinct substances: granosphero- 

 plasm, staining pink, and other cytoplasm, staining blue 

 (fig. i9<?). The outer part of the ray contains cytoplasmic 

 microsomes, while the middle part of the same ray consists 

 of microsomes of the granospheres. The Ehrlich-Biondi 

 stain is thus misleading, as it does not differentiate the 

 granosphere from the cytoplasm; or, if a slight differ- 

 entiation is made, it appears as though the zonal rays 

 surrounding the microcentrum are all, and throughout, of 

 the same quality, which they are not. For the nuclei of 

 the leucocytes the toluidine stain is entirely unsuitable, as it 

 does not differentiate the various nuclear granulations but 

 stains them all alike. 



At times the granosphere, instead of being starlike and 

 consisting of very minute grains of indefinable form, appears 

 to have fallen to pieces, so to say, consisting of a smaller 

 number of larger globular granules, irregularly scattered 

 about, and not close enough together to form a solid sphere, 

 or zone. The microcentrum, which is generally round in 

 outline, is at other times starlike, irregular, or even broken 

 up into several smaller areas, one adjoining the other as in 

 fig. i8<5, which is a toluidine stain. No distinct centrosomes 

 are visible in the leucocyte, as indeed is generally the case 

 with toluidine stains; but there are some shaded portions on 

 the archoplasm which must be considered as corresponding 

 to the somosphere, and which may or may not contain un- 

 stained centrosomes. 



