10 ART. 3. — SEIICHI kawamura: 



manner the fnngus light is affected by a change in environmental 

 temperature. A large test-tube, 80 cc. in capacity, was provided 

 with a wooden stopper with two holes, one large and one small, 

 the former for the insertion of the thermometer and the latter for 

 the purpose of ventilation which seemed to be necessary in order 

 to avoid the accumulation of carbon dioxide produced by respira- 

 tion. Test-tubes were used for the experiments,, as they offered 

 facility in rapidly changing the temperature of the air within. 

 The change of temperature was effected by dipping the test-tube 

 into warm water or into a freezing mixture. A second thermo- 

 meter was placed directly in the warming or the cooling medium 

 for comparing the temperatures inside and outside of the test-tube. 

 After the air in the tube has continued to maintain for some time 

 a certain temperature, the mouth was opened and the luminous 

 object — generally a piece of a gill — was quickly inserted. In 

 numerous experiments made to observe the influence of tempera- 

 ture of water or of various gases on the fungus, nearly the same 

 result was obtained in all cases of each sort, irrespective of 

 whether a whole fungus or a few gills or mere fragments of a gill 

 were used. 



(a) 0°C. This temperature was produced by the use of crushed 

 ice. When a luminous gill was subjected to this temperature, the 

 light commenced to fade after twenty seconds, and in two minutes' 

 time it faded away very markedly, say by about two-thirds of 

 the original intensity. After that the fading took place at a slower 

 rate. In half an hour the change of light intensity became so 

 slow that scarcely any difference could be detected at intervals of 

 several minutes, untill after two hours from the beginning, the 

 light became totally invisible to the eye. After the gill was kept 

 one hour and a half longer in the cooled tube, it was taken out 

 and placed in room temperature of 13°C. Ten minutes afterward, 



