BY R. GREIG-SMITH. 



275 



is comparatively great, and the numbers must therefore not be 

 accepted too rigidly. A difference of two units is sometimes, of 

 one unit frequently obtained. As a rule, the media contained a 

 sugar (2%), a salt (0-2%), asparagin (004-0 06%), and agar 

 (2 %), excepting when a nutrient was under examination. 



With one exception, all the races isolated from nodules which 

 produced a slime upon potato- or bean-agar. also formed it upon 

 the synthetic media. The exception was a race isolated from the 

 Blue Lupin which, while forming slime luxuriantly upon the 

 plant media, could not be induced to form it from any of the 

 combinations of nutrients hereinafter described. 



The presence of a carbohydrate capable of being converted into 

 gum is necessary for the growth of all slime-forming bacteria. 

 Saccharose and maltose are generally the most easily changed, 

 and other sugars and carbonaceous substances may or may not 

 be capable of being utilised by the bacterium. For the sake of 

 comparison, I have in the following table adopted the yield from 

 The Influence of Sugars upon Slime-Formation. 





With citrate of potash. 



With phosphate of soda. 



Expt. 1. 



9/7/04. 



Expt. 2. 



1/11/04. 



Expt. 3. 14/3/06. 



6 cs 

 11 



Sid 



1 1 

 10 



§1 

 10 



'ft g 

 10 



10 



co s-i 



10 



J3 

 10 



.5 

 o 



10 



6 c3 



v 'a 

 * 9 



Si N 



10 



Dextrose 



10 



10 



Levulose 



s 



10 



10 



13 



28 



3 



3 



3 



10 



10 



Maltose 



13 



90 



83 



76 



11 



7 



8 



5 



11 



10 



Saccharose ... 



9 



50 



43 



26 



15 



5 



6 



5 



12 



10 



Mannit 



10 



48 



36 



23 



8 



8 



6 



6 



12 



10 



Glycerin 



10 



13 



13 



13 



18 



8 



o 



3 



10 



2 



Lactose 



1 



— 



— 



— 











5 



2 







1 



Dextrose actual %... 



20 



3 



3 



3 



3 



4 



11 



8 14 j 10 



1 



dextrose as a standard ( — 10), but the actual weight of slitne 

 calculated as being obtained from 100 c.c. of the dextrose medium 

 is also given, and from these the actual weights from all the 

 other sugars may be calculated. 



