BY R. GREIG-SMITH. 



287 



Nodule race (a), short vacuolated cells staining deeply. 

 ., (b), „ „ „ „ feebly. 



,, „ (c), long vacuolated rods. 



,, ,, (d), short and long forms with very many bacteroids. 



Stem race, short vacuolated cells. 



In testing the influence of the various forms of combined 

 carbon, the media contained 006% asparagin, - 2% sodium 

 phosphate, 2 % of sugars, etc., and just before pouring the plates 

 0-1 c.c. of 2N/1 sodium carbonate was added to each tube of 

 molten agar (20 c.c). In this and the succeeding experiment, 

 the slimes were scraped off upon the sixth day; the plates were 

 reinfected and again scraped on the fourteenth day. 



Lupin Eaces with different forms of Carbonaceous Nutrient. 



Dextrose 



Levulose 



Maltose 



Saccharose 



Mannit 



Glycerin 



Lactose 



(a) 



(b) 



(c) 



(d) 



Stem 



(d) 

 neutral 



4 



3 



18 



1 



2 



23 



6 



6 



5 



2 



1 



23 



13 



11 



14 



1 



9 



11 



8 



10 



8 



6 



1 



1 



18 



22 



20 



1 



I 



22 



10 



7 



10 



3 



2 



12 



2 



2 



6 



2 



2 



18 



Lupin Races with different forms of Nitrogenous Nutrient. 



Dextrose, sodium phosphate, 

 and 



Asparagin (, = 10) 



Urea ... 



Peptone 



Potassium nitrate ... 



Ammonium phosphate 



Asparagin, actual %... 

 The same without alkali ) 



(7 days) } - 



(a) 



(b) 



(e) 



(d) 



Stem 



(d) 



neutral 



10 



10 



10 



10 



10 



10 



6 



6 



1 



5 



10 



8 



16 



16 



6 



18 



13 



6 



6 



6 



2 



9 



14 



7 



6 



6 



1 



46 



18 



9 



3 



3 



16 



3 



2 



— 



2 



2 



9 



IS 



1 



21 



It is evident that races " a " and " b " are the same and that 

 "c" and "d" are different. Race " c " differs from "a" and 

 " b " chiefly in being able to form a considerable amount of slime 



