574 CONTRIBUTION TO OUR KNOWLEDGE OF THE ACTION OF RENIN, 



Table ix. . 

 Acidity of milk, 18-8°. 

 5 c.c. milk in each tube. - 15 c.c. rennet in each tube. 



Tubes. 



n Sodium 

 To lactate. 



Eesult. 



1 





Clotting in 27 min. 



2 



0-1 c.c. 



2S „ 



5> 



0-2 c.c. 



28 „ 



4 



3 c.c. 



28 „ 







0-4 c.c. 



28 „ 



6 



0-5 c.c. 



28 ,, 



7 



0-6 c.c. 



2S „ 



8 



- 7 c.c. 



29 ,, 



The pffect of other sodium salts. — The action of two other salts 

 of sodium, di-sodium hydrogen phosphate and sodium chloride, 

 was examined. Both these salts delayed somewhat the clotting, 

 as shown by Tables x. and xi. The addition of increasing- 



Acidity of milk, 18-8°. 

 5 c.c. milk in each tube. 



Table x. 



- 15 c.c. rennet in each tube. 



Tubes. 



fNaCl 



Result. 



1 

 2 

 3 

 4 



0T c.c. 

 0-4 c c. 

 0'S c.c. 



Clotting in 39 min. 

 „51 „ 

 „54 „ 

 ,. 38 „ 



quantities of ^JSTaCl from 0-1 c.c. to OS c.c. to 5 c.c. milk slowed 

 somewhat coagulation. With sodium phosphate the addition of 

 quantities from Ol c.c. to 07 c.c. ■—■ Na„HP0 4 delayed the speed 

 of clotting. 



Causation of the prevention of clotting. — In the first place it 

 was desirable to ascertain whether the rennet had been destroyed 

 or whether its action upon the milk had been inhibited. It has 



