576 CONTRIBUTION TO OUR KNOWLEDGE OF THE ACTION OF RENIN, 



When, however, in addition to hydrochloric acid, rennet was 

 added, clotting occurred in a few minutes. Experiments in this 



Table xii. 

 Acidity of milk, 16-8°. 

 5 c.c. milk in each tube. 0*15 c.c. rennet in each tube. 







Time in con- 





Tube. 



V^NaOH. 



tact without 



Remarks. 







clotting. 





1 



0-4 c.c. 



270 min. 



added 0"3 c.c^HCl. No clotting. 



2 



0'5 c.c. 



270 ,, 



added 0-4 c.c. T ^HC1. ,, 



3 



0-6 c.c. 



270 ,, 



added 4 c.c. T N oHCl. ,, 



4 



- 5 c.c. 



270 „ 



added 0-4c.c. T N oHCl. + 1 c.c.|CaCl. 2 . 

 No clotting. 



5 



0-5 c.c. 



250 „ 



added 0'4 c c.^HCl. + l c.c.fCaCl 2 . 

 No clotting. 



6 



0-6 c.c. 



250 ,, 



added 0"6 c c. T ^HCl. + 1 c.c.^CaCl 2 

 + - 5 c.c. fresh milk. No clotting. 



category are tabulated in Table xiii. 



The conclusion to be drawn from these data is that the presence 



Table xiii. 



5 c.c. 



milk in each tube. 





Tube 



T N oNaOH 



Time in con- 

 tact without 

 clotting. 



Remarks. 



1 

 2 

 3 

 4 

 5 



0-4 c.c. 

 0-7 c.c. 

 0-6 c.c. 

 0'6 c.c. 

 0-6 c.c. 



270 min. 

 270 ,, 

 300 ,, 

 270 ,, 

 250 ,, 



added 0"3 c.c. ^.HCl + 0'15 c.c. rennet. 



Clot in 5 min. 

 added 0'6 c.c. T N _HC1 + 0-15 e.c. rennet. 



Clot in 86 min. 

 added 5 c.c. ^HCl-f- 1 c.c. NCaCl 2 + 



- 15 c.c. rennet. Clot in 4 min. 

 added 0'5 c.c. T N_HC1 + 1 c.c. NCaCl. 2 + 



015 c.c. rennet. Clot in IS min. 

 added 0"6 c.c. n HC1+1 c.c. NCaCl.,+ 



10 5 ^ 



5 c.c. milk + 0'15 c.c. rennet. Clot 

 in 26 min. 



of small quantities of soda insufficient to neutralise the milk 

 leads to the destruction of the added rennet. 



