490 Drs. A. Fick and J. Wislicenus on the 



(a) Fick : total quantity of urine 790 cubic centims. In 10 

 cubic centims. we found 0*0619 grm. of common salt, and, re- 

 gard being had to the necessary corrections*, 0*1580 grm. of 

 urea. The total quantity of the latter amounted, consequently, 

 to 12-4820 grms. 



(b) Wislicenus : quantity of urine 916 cubic centims. In 10 

 cubic centims. 0*03 grm. of salt and 0*1284 grm. of urea; in 

 the whole quantity, therefore, 11*7614 grins, of the latter. 



II. Work urine, from half-past 5 in the morning of the 30th 

 of August till 20 minutes past 1. 



(a) Fick: quantity of urine 396 cubic centims.; pale yellow, 

 clear, acid. In 10 cubic centims. 0*0395 grm. of salt and 0*1776 

 grm. of urea. Total quantity of urea 7*0330 grms. 



(b) Wislicenus : quantity of urine 261 cubic centims. ; pale 

 yellow, turbid after cooling, acid. In 10 cubic centims. 0*0460 

 grm. of salt and 0*2566 grm. of urea. Total quantity of urea 

 6*6973 grms. 



III. The after-work urine, from 20 minutes past 1 on the 30th 

 of August till 7 in the evening. In both cases darkish yellow, 

 a sediment being deposited on cooling, acid. 



(a) Fick: quantity of urine 198 cubic centims. In 10 cubic 

 centims. 0*007 grm. of salt and 02612 grm. of urea. Total 

 quantity of urea 5*1718 grms. 



(b) Wislicenus : quantity of urine 200 cubic centims. In 10 

 cubic centims. 0*018 grm. of salt and 0*2551 grm. of urea. Total 

 quantity of urea 5*1020 grms. 



It is evident that the amount of urea found cannot be the 

 correct measure for the quantity of oxidized albuminoid sub- 

 stances, particularly as a portion of nitrogen, which must not be 

 omitted, must always have been present in the sediment, which 

 consisted almost exclusively of acid urate of soda. It therefore 

 became necessary to ascertain the total quantity of nitrogen 

 present. The urea determinations were undertaken principally 

 in order that, if any accident should happen to any of the spe- 

 cimens of urine on their way to Zurich, there might be at 

 least some data saved for an approximate calculation of the 

 nitrogen. 



The absolute nitrogen determinations were made between the 

 4th and 6th of September, in the laboratory of the Zurich Uni- 

 versity, with the urine which had remained perfectly fresh. 5 

 cubic centims. of it were distilled with excess of soda-lime, from 

 a suitable apparatus, into pure hydrochloric acid, the residue 

 heated till it became colourless, and air drawn through the ap- 

 paratus and through the acid. The determination of the am- 



* Neubauer and Vogel 3 Analyse des Karnes, 4 Aufl. p. 143-146. 



