GENERAL PLAN OF INQUIRIES PROSECUTED. 5 



F. Belationships. 



33. Do these fish go in schools after they have done spawning, or 

 throughout the year, or are they scattered and solitary ? 



34. Have they any special friends or enemies % 



35. To what extent do they prey on other fish ; and on what species ? 



36. To what extent do they suffer from the attacks of other fish, or 

 other animals ? 



G. Food. 



37. What is'the nature of their food ? 



38. Are there any special peculiarities in the manner of feeding of 

 these fish ? 



39. What amount of food do they consume'? 



H. Eeproduction. 



40. Is there any. marked change in the shape or color of either sex 

 during the breeding-season, or any peculiar development of, or on, any 

 portion of the body, as the mouth, fins, scales, &c. ? 



41. Are there any special or unusual habits during the spawning- 

 season ? 



42. Is spawning interfered with by lines or nets, or otherwise ? 



43. At what age does the male begin to breed, and at what age the 

 female ? 



44. For how many years can these fish spawn ? 



45. Does the act of spawning exert an injurious effect ? 



46. Where do these fish spawn, and when 1 ? 



47. Can you give any account of the process, whether males and 

 females go in pairs, or one female and two males $ whether the sexes are 

 mixed indiscriminately, &c. ? 



48. Is the water ever whitened or colored by the milt of the male ? 



49. What temperature of water is most favorable for hatching ? 



50. At what depth of water are the eggs laid, if on or near the 

 bottom % 



51. What is the size and color of the spawn ? 



52. What is the estimated number for each fish; and how ascer- 

 tained ? 



53. Answer the question for one season, and for the lifetime. 



54. Do the eggs, when spawned, sink to the bottom, and become 

 attached to stones, grass, &c. ; or do they float in the water until 

 hatched f 



55. Do the fish heap up or construct any kind of nest, whether of 

 sand, gravel, grass, or otherwise ; and if so, is the mouth, the snout, or 

 the tail used for the purpose, or what ; and if so, how is the material 

 transported ; or do they make any excavation in the sand or gravel "? 



56. Do they watch over their nest, if made either singly or in pairs ? 



57. When are the eggs hatched, and in what period of time after being 

 laid? 



58. What percentage of eggs laid is usually hatched ? 



59. What percentage of young attains to maturity ? 



60. What is the rate of growth % 



61. Do the parents, either or both, watch over the young after they 

 are hatched ? 



62. Do they carry them in the mouth or otherwise ? 



63. What enemies interfere with or destroy the spawn or the young 

 fish ; do the parent fish devour them ? 



