364 Dr. W. E. Adeney on the 



surface-layer to a depth of 50 mm. for 48 hours. After 

 this, a sample was drawn from the bottom of the open 

 limb, and one from the lower portion of the closed limb, 

 about 200 mm. from the bottom. The gases were extracted 

 from these and the nitrogen in each determined, with the 

 following results : — 



Open Limb. Closed Limb. 



12-12 1-49 



Six months later another sample was collected from the lower 

 portion of the closed limb, as before, and the nitrogen in 

 it determined ; it was 1*73 



Experiments ivith Broken Surfaces. 



The experiment No. 1, and several others which were made 

 during the earlier part of this investigation, but which it is 

 unnecessary here to record, since the results were similar, 

 shows that a retardation of solution takes place at the surface 

 of the water exposed to the air. Experiments were accordingly 

 made in wdrich the surfaces of the water exposed to the air 

 were kept continuously broken. 



No. 4. — A tube 1100 mm. long and 50 mm. diameter was 

 filled with nitrogen-free sea- water, and immersed in the 

 water-jacket to a depth of 1025 mm. A glass stirrer of a 

 gridiron shape, 40 mm. broad, was fixed to dip 25 mm. 

 below the surface of the water, and was mechanically 

 rotated for 19 hours. At the end of this time the tem- 

 perature of the upper layer of the water-column above 

 the water-jacket was 8°*3 C, and that of the water-jacket 

 7°'8 C. Samples were drawn form the top and bottom 

 layers of the water-column, and the nitrogen determined 

 in each, with the following results : — 



Top layer. Bottom layer. 



12-69 12-45 



At commencement. 1*51 



No. 5. — A similar experiment was made with distilled water, 

 with results as follows : — 



13-29 11-02 



At commencement. 1*46 



Sea-water saturated at 8°*3 C. contains 12*91 c.c. No 

 per litre. Distilled water saturated at 8°'3 0. contains 

 15*92 c.c, No per litre. 



