444 BOTANICAL GAZETTE [june 



these cells has actually divided or has merely elongated. The 

 nuclei of cells i, 2, and 3 of the carpogonial branch are often not 

 in the resting condition, that is, all the chromatin is not in the 

 nucleolus. The chromatin in these nuclei may be in one body sur- 

 rounded by a number of small granules (fig. 32, cells 1, 2; fig. 38, 

 cell 1), or in several small bodies (fig. 32, cell 3; fig. 36. cell 3; fig. 

 37, cell 3). The 3 terminal cells (4-6) are smaller than the first 

 3 or 4 cells and their nuclei are generally in the resting state. In 

 many of the Delesseriaceae and Ceramiaceae some of the cells of 

 the carpogonial branches contain two or more nuclei. The veg- 

 etative cells in these forms are multinucleate, and it is not surprising 

 that this nuclear condition should occur also in cells of the carpo- 

 gonial branches. In a form like Dumontia, where nearly all the 

 vegetative cells are uninucleate, it is surprising that any cells of 

 the carpogonial branch should contain more than one nucleus. 

 However, the cytoplasmic contents of the carpogonial cells are 

 much greater than those of the adjoining vegetative cells in propor- 

 tion to their size, and the presence of an extra amount of chromatin 

 in the larger cells of the carpogonial branches is quite in accord with 

 the current belief of a definite relation in volume between cell and 

 nucleus. The mature carpogonium lies close to or in contact with 

 the third cell (rigs. 32-38). 



Only 4 trichogynes with spermatia attached to them were 

 found in all the material examined. These were found in the 

 material collected in April 191 5. Although this number is small, 

 it is not less than would be expected, since only a very few tricho- 

 gynes were found projecting beyond the surface of the thallus. 

 This may have been due to the fact that the mature trichogynes 

 persist for only a short time, or that they are easily broken off. 

 It is to be regretted that none of these trichogynes with the sper- 

 matia attached to them could be traced back to the carpogonium. 

 In none of these cases was it possible to find even the carpogonium. 

 In one case one spermatium had fused with the tip of a trichogyne, 

 while 7 others were merely adhering to its sides (fig. 41). Judging 

 from the way it stained, the cytoplasm in this one spermatium 

 and in the tip of the trichogyne had begun to disintegrate. The 

 other spermatia stained very lightly, and it was not possible to 



