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MANUAL OF DETERMINATIVE BACTERIOLOGY 



Potato: Thick, pale yellow becoming 

 dark brown layer, slimy. The medium 

 becomes bluish-gray. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 37°C. 



Pathogenic for mice. 



Source: Regarded by Jaeger as the 

 cause of Weil's disease (infectious jaun- 

 dice) as it was found repeatedly in 

 patients suffering from this disease. 

 See Leptospira icterohaemorrhagiae. 



Habitat: Water. 



3. Pseudomonas fluorescens Migula. 

 (Bacillus fluorescens liquefaciens Flugge, 

 Die Mikroorganismen, 18S6, 289; Migula, 

 in Engler and Prantl, Die natiirl. Pflan- 

 zenfamilien, 1, la, 1895, 29; Bacterium 

 fluorescens Lehmann and Neumann, Bakt. 

 Diag., 1 Aufl., .2, 1896, 272.) From Latin, 

 fluor, flowing; M.L. fluoresco, to fluoresce. 



Rods: 0.3 to 0.5 by 1.0 to 1.8 microns, 

 occurring singly and in pairs. Motile, 

 possessing a polar flagellum. Gram- 

 negative. 



Gelatin colonies: Circular, with green- 

 ish center, lobular, liquefying quickly. 



Gelatin stab : Infundibuliform lique- 

 faction, with whitish to reddish-gray 

 sediment. 



Agar slant : Abundant, reddish layer, 

 becoming reddish-gray. The medium 

 shows greenish to olive-brown coloration. 



Broth: Turbid, flocculent, with yellow- 

 ish-green pellicle and grayish sediment. 



Litmus milk: No coagulation; becom- 

 ing alkaline. 



Potato: Thick, grayish-yellow, spread- 

 ing, becoming light sepia-brown in color. 



Indole is not formed. 



Nitrates reduced to nitrites and am- 

 monia. 



Acid from glucose. 



Blood serum liquefied. 



Aerobic. 



Optimum temperature 20° to 25°C. 



Not pathogenic. 



Source: Water, sewage, feces. 



Habitat: Soil and water. 



4. Pseudomonas viscosa (Frankland 

 and Frankland) Migula. (Bacillus vis- 

 cosKs G. and P. Frankland, Ztschr. f. 

 Hyg., 6, 1889, 391 ; Migula, Syst. d. Bakt., 

 2, 1900, 900.) From M. L. viscidus, 

 sticky, viscid. 



Small rods: 0.5 by 1.5 to 2.0 microns, 

 occurring singly. Motile and presum- 

 ably polar flagellate. Gram-negative. 



Gelatin colonies: Grayish, granular, 

 with fimbriate margin. Medium assumes 

 a green fluorescent color around each 

 colony. 



Gelatin stab: Infundibuliform lique- 

 faction. Liquefied portion green fluores- 

 cent with greenish-white pellicle. 



Agar slant: Thin, greenish-white, the 

 medium becoming greenish. 



Broth: Turbid, with greenish pellicle. 



Litmus milk: Not coagulated. 



Potato : Moist, chocolate-brown, viscid. 



Indole not formed. 



Nitrites not produced from nitrates. 

 Destroys nitrate with the production of 

 ammonia. 



Aerobic, facultative. 



Distinctive characters : Resembles 

 Pseudomonas fluorescens except that 

 growth on agar, gelatin and potato is 

 viscid. 



Optimum temperature 20°C. 



Source: Unfiltered water from Kent, 

 England. Common. 



Habitat: Water. 



5. Pseudomonas fairmountensis 



(Wright) Chester. (Bacillus fairmoun- 

 tensis Wright, Memoirs Nat. Acad. 

 Sci., 7, 1895, 458; Chester, Man. 

 Determ. Bact., 1901 , ill ; Achrojuobacler 

 Jairmountense Bergey et al., Manual, 

 1st ed., 1923, 146.) From M. L. of Fair- 

 mount Park (Philadelphia.) 



Medium-sized rods, occurring singly, 

 in pairs and in chains. Motile, posses- 

 sing polar flagella. Gram-negative. 



Gelatin colonies : Circular, white, 

 translucent. Dark centers with a green- 

 ish shimmer, thinner edges and faint 

 radial lines. 



Gelatin stab : Crateriform liquefaction. 



