FAMILY PSEUDOMONADACEAE 



91 



Agar slant : Grayish-white, glistening. 

 Broth: Turbid. 



Litmus milk : Alkaline, litmus reduced. 

 Potato: Raised, granular, spreading, 

 viscid. 

 Indole is formed. 



Nitrites not produced from nitrates. 

 Aerobic, facultative. 

 Optimum temperature 20° to 25°C. 

 Habitat: Water. 



6. Pseudomonas ureae Bergey et al. 

 (Culture No. 3 of Rubentschick, Cent, f . 

 Bakt., II Abt., 72, 1927, 101; Bergey et 

 al., Manual, 3rd ed., 1930, 173.) From 

 Greek, urum, urine; M. L., urea, urea. 



Rods: 0.6 to 0.7 by 1.7 to 2.0 microns, 

 occurring singly and in pairs. Motile. 

 Gram-positive. 



Gelatin stab : Infundibuliform lique- 

 faction. 



Agar colonies: Circular, grayish-white. 



Agar slant : Grayish-white layer be- 

 coming greenish-fluorescent. 



Broth: Turbid. 



Litmus milk: Peptonized. 



Potato: Yellowish-brown streak. 



Indole not formed. 



Nitrates reduced with gas formation. 

 Ammonia formed. 



Urea attacked. 



Hydrogen sulfide formed. 



Methylene blue reduced. 



Aerobic, facultative. 



Can grow at 0° C. 



Optimum temperature 20°C. 



Habitat : Sewage filter beds. 



This species is included here through 

 an oversight. It should have been 

 placed in the Appendix to the genu.s 

 Pseudomonas as the original description 

 is too incomplete to determine its real 

 nature. It is reported to be Gram-posi- 

 tive and motile; but the number and ar- 

 rangement of flagella are not given. If 

 it really is Gram-positive, the species is 

 probably peritrichous and does not l)e- 

 long in Pseudomonas. 



7. Pseudomonas pavonacea Levine 

 and Soppeland. (Bui. No. 77, Iowa 



State Agricultural College, 1926, 41.) 

 From Latin, pavo, peacock. 



Rods : 0.5 by 4.5 microns, with truncate 

 ends, occurring singly and in chains. 

 Motile. Gram-negative. 



Gelatin stab: Crateriform liquefaction. 

 Medium becoming brown. 



Agar colonies: Circular, raised, becom- 

 ing green, amorphous, entire. 



Agar slant : Greenish, smooth, glisten- 

 ing, viscid, medium becoming slightly 

 brown. 



Broth: Turbid, with viscid sediment. 

 Medium turned dark brown. 



Litmus milk: Slightly alkaline. Lit- 

 mus reduced. Peptonized after 10 days. 



Potato: No growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



Blood serum liquefied in 5 days. 



No acid or gas from carbohydrate 

 media. 



Aerobic, facultative. 



Optimum temperature 22°C. 



Source: Isolated from activated 

 sludge. 



8. Pseudomonas effusa Kellerman et 

 al. (Kellerman, McBeth, Scales and 

 Smith, Cent. f. Bakt., II Abt., 39, 1913, 

 515; also Soil Science, 1, 1916, 472; Cel- 

 Udomonas effusa Bergey et al.. Manual, 

 1st ed., 1923, 162; Bacillus effusus ap- 

 pears first as a synonym in Bergey at al., 

 ibid. ; later used as name of species 5th 

 ed., 1939, 610.) From Latin, effusus, ef- 

 fuse, spread out. 



Rods: 0.4 by 1.7 microns. Motile 

 with one to three polar flagella. Gram- 

 negative. 



Gelatin stab: T^iquefaction. 



Agar slant: Luxuriant, glistening, 

 moist, creamy growth. Greenish fluo- 

 rescence. 



Peptone starch agar slant: Abundant, 

 flat, moist rich creamy growth. Medium 

 shows greenish fluorescence. 



Broth: Turbid. 



Litmus milk: Alkaline. Coagulation 

 and digestion. 



