92 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Potato: Rich, creamy spreading 

 growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Ammonia is produced. 



Acid from glucose, maltose, starch, 

 glycerol and mannitol. No acid from 

 lactose or sucrose. 



Cellulose is attacked. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Isolated from soils in Utah. 



Habitat: Soil. 



8a. Psendomonas effusa var. non-lique- 

 faciens Kellerman et al. (loc. cit.). A 

 non-liquefying variety that acts more 

 slowly on litmus milk. 



Source: Soils from Utah. 



9. Pseudomonas reptilivorous Cald- 

 well and Ryerson. (Jour. Bact., 39, 1940, 

 335.) From Latin, reptile, a reptile and 

 voro, to devour, destroy. 



Rods: 0.5 by 1.5 and 2.0 microns, oc- 

 curring singly, in pairs and in short chains 

 and having rounded ends. Actively 

 motile with two to six polar flagella. 

 Gram-negative. 



Gelatin colonies : After 24 hours, small, 

 circular, smooth, entire. Liquefaction 

 with a yellowish-green fluorescence. 



Gelatin stab : Infundibuliform lique- 

 faction becoming stratiform. Putrid odor 

 present. 



Agar cultures: Circular, smooth, glis- 

 tening, slightly raised, butyrous, translu- 

 cent, 2 mm in diameter. 



Agar slant: Growth abundant, smooth, 

 filiform, glistening, butyrous and translu- 

 cent. 



Broth: Turbid with pellicle and sedi- 

 ment. Putrid odor. 



Litmus milk: Alkaline, peptonization, 

 complete reduction. Disagreeable odor. 



Potato: Growth moderate, spreading, 

 glistening, yellowish-gray to creamy. 

 Disagreeable odor. Medium becomes 

 brownish-gray. 



Indole not formed. 



Nitrates not produced from nitrates. 



Hydrogen sulfide not produced. 



Slightly acid, becoming alkaline in 

 glucose. No acid from arabinose, xylose, 

 lactose, sucrose, maltose, trehalose, raf- 

 finose, mannitol, dulcitol, inositol and 

 salicin. 



Starch not hydrolyzed. 



Pathogenic for guinea pigs and rabbits, 

 horned lizards, Gila monsters and chuck- 

 wallas. 



Temperature relations : Optimum 20° 

 to 25°C. Maximum 37°C. 



Distinctive characters : Yellowish- 

 green fluorescence present in meat in- 

 fusion media. Pathogenic. 



Source : Isolated in a bacterial disease 

 of horned lizards and Gila monsters. 



Habitat: Pathogenic for lizards. 



10. Pseudomonas syncyanea (Ehren- 

 berg) Migula. {Vibrio syncyaneus Eh- 

 renberg, Berichte ii.d. Verh. d. k. Preuss. 

 Akad. d. Wissensch. z. Berlin, 5, 1840, 

 202 ; Vib7-io cyanogen.es Fuchs, Magazin fiir 

 die gesamte Tierheilkunde, 7, 1841, 190; 

 Bacillus syncyaneus Schroeter, Krypto- 

 gam. Flora von Schlesien, S, 1 , 1886, 157 ; 

 Bacillus cyanogenus Zopf, Die Spalt- 

 pilze, 3 Auk., 1885, 86; Migula, in Engler 

 and Prantl, Die nattirl. Pflanzenfam., 

 /, la, 1895, 29; Bacterium syncyaneum 

 Lehmann and Neumann, Bakt. Diag., 1 

 Aufl., 2, 1896, 275; Pseudomonas cyano- 

 genes Holland, Jour. Bact., 5, 1920, 224.) 

 From Greek, syn, with; kyaneos, dark 

 blue, dark. 



Rods with rounded ends, occurring 

 singly, occasionally in chains, 0.7 by 2.0 

 to 4.0 microns. Motile with two to four 

 polar flagella. Gram-negative. 



Gelatin colonies: Flat, bluish, trans- 

 lucent. 



Gelatin stab : Surface growth shiny, 

 grayish blue. The medium is colored 

 steel-blue with greenish fluorescence. 

 Gelatin is liquefied. Some strains do not 

 liquefy. 



Agar slant : Grayish-white streak. The 

 medium takes on a bluish-gray color with 

 slight fluorescence. 



