FAMILY PSEUDOMONADACEAE 



93 



Broth: Turbid mth marked fluo- 

 rescence. 



Litmus milk: Unchanged. In associa- 

 tion with lactic acid bacteria the milk 

 takes on a deep blue color. 



Potato: Yellowish-gray, shiny layer, 

 becoming bluish-gray. The medium be- 

 comes bluish-gray. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 25°C. 



Habitat: The cause of blue milk. 



11. Pseudomonas schuylkilliensis 



Chester. (Bacillus fluorescens schuyl- 

 killiensis Wright, ]\Iemoirs, Xatl. Acad. 

 Sci., 7, 1895, 448; Chester, Determinative 

 Bact., 1901, 320.) From :\I. T>. of the 

 Schuylkill (River). 



Synonyms: Pseudomonas capsulala 

 Chester, Man. Dctcrm. Bact., 1901, 322 

 (Bacillus fluorescens capsulalus Pottien, 

 Ztschr. f. Hyg., 11, 1896, 140); Pseudo- 

 monas dennatogenes Fuhrmann, Cent, 

 f. Bakt., II .\bt., 17, 1906, 356. 



Short rods, with rounded ends, occur- 

 ring singly, in pairs and in chains. Mo- 

 tile, possessing a polar flagellum. Gram- 

 negative. 



Gelatin colonies: Grayish-white, trans- 

 lucent, with brownish center, radiate 

 margin, becoming bluish-green. 



Gelatin stab: Slow crateriform lique- 

 faction, with blue-green fluorescence. 



Agar slant : Grayish, translucent 

 growth. Medium shows greenish fluores- 

 cent. 



Broth: Turbid, with slight pellicle and 

 blue-green fluorescence. Stringy sedi- 

 ment. 



Litmus milk: Coagulated, with slow 

 reduction of litmus; peptonized. 



Potato: Brownish, spreading, viscid, 

 thick. 



Indole is formed (trace). 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Does not grow at 35° to 36°C. 



Source : Isolated from Schuylkill River 

 water. 

 Habitat: Water. 



12. Pseudomonas chlororaphis (Guig- 

 nard and Sauvageau) Bergey et al. (Ba- 

 cillus chlororaphis Guignard and Sauva- 

 geau, Compt. rend. Soc. Biol. Paris, 1, 

 10 s6r., 1894, 841;. Bergey et al., Manual, 

 3d ed., 1930, 183; also see Lasseur and 

 Dupaix-Lasseur, Trav. Lab. Microbiol. 

 Fac. Pharm. Nancy, Fasc. 9, 1936, 35.) 

 From Greek, chloros, greenish yellow; 

 r aphis, needle. 



Rods: 0.8 by 1.5 microns, with rounded 

 ends, occurring singly and in pairs. Mo- 

 tile with polar flagella. Gram-negative. 



Gelatin colonies: Circular, viscid, 

 transparent, glistening, lobate margin, 

 with fluorescent corona. Dissociates 

 readily (Lasseur and Dupaix-Lasseur, 

 loc. cit.). 



Gelatin stab: Stratiform liquefaction. 



Broth: Turbid, fluorescent, with crys- 

 tals of green, water-soluble chloro- 

 raphine. 



Litmus milk : Coagulation. Peptoniza- 

 tion. Crystals of chlororaphine form 

 in the central part of the culture. 



Potato: Citron-yellow layer. Crystals 

 of chlororaphine are formed. 



Nitrates reduced to nitrites. 



Indole not formed. 



Pigment formation: Asparagine, potas- 

 sium phosphate, glycerol, sulfate of mag- 

 nesium and sulfate of iron are indispensa- 

 ble to the formation of crystals of 

 chlororaphine. 



Aerobic, facultative. Optimum tem- 

 perature 25° to 30°C. 



Pathogenic for laboratory animals. 

 Exotoxin formed. 



Habitat: Water. 



13. Pseudomonas myxogenes Fuhr- 

 mann. (Cent. f. Bakt., II Abt., 17, 

 1907, 356.) From Greek, myxa, mucus; 

 gennao, to beget; M. L. slime producing. 



Rods: 0.4 to 0.5 by 1.0 to 1.5 microns, 

 occurring singly and in pairs. Motile, 

 possessing a bundle of five to seven polar 

 flagella. Gram-negative. 



