94 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Gelatin colonies: Smooth, soft, flat, 

 spreading, entire, yellowish-green. 



Gelatin stab: Growth along stab. 

 Liquefaction with yellowish-white sedi- 

 ment. 



Agar colonies : Circular, raised, smooth, 

 amorphous, entire. 



Agar slant: Yellowish-white, moist, 

 glistening, becoming light green-fluores- 

 cent. 



Broth: Turbid, with yellowish-white 

 sediment. 



Litmus milk: Flocculent precipitation. 

 Slow peptonization with yellow serum. 

 Alkaline. 



Potato : Dirty yellow to olive, moist, 

 glistening, entire. 



Indole is formed. 



Nitrates reduced to nitrites and am- 

 monia. No gas formed. 



Aerobic, facultative. 



Optimum temperature 22°C. 



Source: Isolated from beer. 



14. Pseudomonas septica Bergey et al. 

 {Bacillus fluorescens septicus Stutzer 

 and Wsorow, Cent. f. Bakt., II Abt., 71, 

 1927, 113; Bergey et al.. Manual, 3rd ed., 

 1930, 169.) From Greek, septikos, putre- 

 factive, septic. 



Rods: 0.6 to 0.8 by 0.8 to 2.0 microns, 

 occurring singly. Motile with a polar 

 flagellum. Gram-negative. 



Gelatin stab: Infundibuliform lique- 

 faction. 



Agar colonies: Circular with opalescent 

 center and transparent periphery. 



Agar slant : Moderate, undulate margin. 



Broth: Turbid with fragile pellicle, 

 greenish in upper poution. 



Litmus milk: Alkaline, coagulated. 



Blood serum not liquefied. 



Acid from glucose. 



Aerobic, facultative. 



Optimum temperature 20^0. 



Habitat: Disease of caterpillars. 



15. Pseudomonas boreopolis Gray and 

 Thornton. (Gray and Thornton, Cent. 

 f. Bakt., II Abt., 73, 1928, 74.) From 



Greek, boreas, the North wind; polis, 

 city; M. L. North City. 



Rods: 0.5 to 1.0 by 2.0 to 3.0 microns, 

 occurring singly and in pairs. Motile 

 with one to five polar flagella. Gram- 

 negative. 



Gelatin colonies : Liquefied. 



Gelatin stab : Liquefied. Medium red- 

 dened. 



Agar colonies: Circular or amoeboid, 

 white to buff, flat to convex, smooth, 

 glistening, translucent border. 



Agar slant: Filiform, whitish, raised, 

 smooth, glistening, fluorescent. 



Broth: Turbid. 



Nitrates reduced to nitrites. 



Starch not hydrolyzed. 



Acid produced from glucose. 



Attacks naphthalene. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Habitat: Soil. 



16. Pseudomonas smaragdina Migula. 

 (Bacillus S7naragdimis foetidus Rei- 

 man, Inaug. Dissertation, Wiirzburg, 

 1887;Migula,Syst.d. Bakt.,.?, 1900, 890.) 

 From Greek, smaragdinas, green like the 

 smaragdus, the emerald. 



Small rods, occurring singly. Non- 

 motile. Gram -negative. 



Gelatin colonies : Small, convex, irregu- 

 lar, whitish with greenish shimmer. 



Gelatin stab: Slight surface growth. 

 Infundibuliform liquefaction. The 

 liquefied medium becomes light emerald 

 green in color. 



Agar colonies: Small, brownish-yellow, 

 convex. 



Agar slant : Abundant growth with 

 greenish fluorescence. 



Broth: Turbid. 



Litmus milk: Not coagulated. 



Potato: Dark brown, becoming choco- 

 late brown. 



Indole not formed. 



Nitrates not reduced. 



The cultures give off an odor resembling 

 jasmine. 



Aerobic, facultative. 



