FAMILY PSEUDOMONADACEAE 



95 



Optimum temperature 37°C. 



Subcutaneous and intravenous inocula- 

 tions into rabbits cause death in 36 to 48 

 hours. 



Source : Isolated from nasal secretions 

 in ozena. 



17. Pseudomonas chlorina (Frankland 

 and Frankland) Levine and Soppeland. 

 {Bacillus chlorinus G. and P. Frankland, 

 Philos. Trans. Roy. Soc. London, 178, 

 1887, 274; Bacterium chlorinum Migula, 

 Syst. d. Bakt., 2, 1900, 471; Levine and 

 Soppeland, Bui. No. 77, Iowa State Agri- 

 cultural College, 1926.) From Greek, 

 chloros, greenish yellow. 



Piods : 0.5 by 1 .5 micron, occurring singly 

 and in short chains. Non-motile. Gram- 

 negative. 



Gelatin stab : Crateriform liquefaction 

 with green fluorescence. Lemon yellow 

 sediment. 



Agar colonies : Circular, raised, smooth, 

 amorphous, entire, becoming greenish 

 yellow. 



Agar slant : Slightly raised, glistening, 

 the medium becoming light greenish 

 yellow. 



Broth : Moderate turbidity. Dirtj' 

 yellow sediment. No pellicle. 



Litmus milk: Peptonized. Litmus re- 

 duced. 



Potato: Scant, olive green growth. 



Indole formed. 



Nitrites produced from nitrates. 



Starch hydrolyzed. 



Blood serum liquefied in .5 days. 



Acid from glucose. 



Aerobic, facultative. 



Optimum temperature 22°C. 



Source : Air. 



18. Pseudomonas oleovorans Lee and 

 Chandler. (Jour. Bact., 41, 1941, 378.) 

 From M. L. oil destroying. 



Short rods: 0.5 by 0.8 to 1.5 microns, 

 occurring singly and in pairs. . Motile. 

 Gram-negative. 



Gelatin stab : No liquefaction after 6 

 weeks. 



Gelatin colonies: Up to 1 mm. in diam- 

 eter, fluorescent ; similar to agar colonies. 



Surface agar colonies : After 24 hours 

 1 to 2 mm. in diameter, smooth, convex, 

 shin3% opaque, creamy, fluorescent by 

 transmitted light. Edge entire in young 

 colonies. 



Deep agar colonies: 0.5 by 1.0 to 1.5 

 mm., lens-shaped, buff-colored, not flu- 

 orescent. 



Agar slant : Growth raised, smooth, 

 fluorescent, edge erose. 



Broth : After 24 hours moderate turbid- 

 ity with slight yellowish viscid sediment. 

 No pellicle or ring. 



Litmus milk: No change. 



Indole not formed. 



Potato : Good growth. 



Nitrites are produced from nitrates. 



Starch is hydrolyzed. 



No acid from glucose, lactose, sucrose, 

 galactose, xylose, mannitol, salicin and 

 glycerol. 



Equally good growth at 25° and 37°C. 



Aerobic. 



Distinctive character: The fluorescent 

 quality of the colonies is not imparted to 

 any of the artificial media used. 



Source : Isolated from cutting com- 

 pound (oil-water emulsion) circulating 

 in a machine shop. The oil in this 

 compound may be utilized as a sole source 

 of energy. 



Habitat : Probably oil-soaked soils. 

 Abundant in cutting compounds. 



19. Pseudomonas incognita Chester. 

 {Bacillus Jiuorescens incognilus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 436; 

 Chester, Determinative Bacteriology, 

 1901, 323.) From Latin, in, not; cogito, 

 to think; M. L. unknown. 



Short rods, with rounded ends, occur- 

 ring singly, in pairs and in chains. Mo- 

 tile, possessing a polar flagellum. Gram- 

 negative. 



Gelatin colonies: Thin, transparent, 

 slightly granular, becoming greenish. 

 Margin undulate. The medium assumes 

 a blue-green fluorescence. 



