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MANUAL OF DETERMINATIVE BACTERIOLOGY 



Gelatin stab : No liquefaction. 



Agar slant: Thin, moist, translucent, 

 becoming greenish. 



Broth: Turbid, with pellicle, becoming 

 greenish. 



Litmus milk: Slightly acid in a month. 

 The litmus is slowly reduced. 



Potato: Moist, glistening, spreading, 

 brown. 



Indole is formed (trace). 



Nitrites are produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 35°C. 



Habitat: Water. 



20. Pseudomonas convexa Chester. 

 {Bacillus fluorescens convexus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 438; 

 Chester, Determinative Bacteriology, 

 1901, 325.) From Latin, convexus, con- 

 vex, arched. 



Short, thick rods, with rounded ends. 

 Motile, possessing a polar flagellum. 

 Gram -negative. 



Gelatin colonies: Circular, convex, 

 glistening, bright greenish, translucent. 

 The medium becomes blue-green, flu- 

 orescent. 



Gelatin stab: Light green, raised, 

 gUstening surface growth. No liquefac- 

 tion. 



Agar slant : Moist, translucent, glisten- 

 ing, light greenish. The medium as- 

 sumes a greenish color. 



Broth: Turbid, becoming greenish. 



Litmus milk: No coagulation; alkaline. 



Potato: Pale brown, spreading. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 30°C. 



Habitat: Water. 



21. Pseudomonas mildenbergii Ber- 

 gey et al. (Der blaue bacillus, Milden- 

 berg. Cent. f. Bakt., II Abt., 56, 1922, 

 309; Pseudomonas cyanogena Bergey et 

 al.. Manual, 1st ed., 1923, 129; not Bacil- 

 lus cyanogenes Flugge, Die Mikroorgan- 



ismen, 1886, 201 ; not Pseudomonas cyano- 

 genes Hammer, Dairy Bact., 1928, 70; 

 Bergey et al.. Manual, 3rd ed., 1930, 

 172.) Named for Mildenberg who first 

 isolated this species. 



Rods: 0.3 to 0.5 by 1.0 to 3.5 microns, 

 with rounded ends, occurring singly. 

 Motile, possessing polar flagella. Gram- 

 negative . 



Gelatin colonies: Circular, lobed, 

 smooth, glistening, slightly raised, steel- 

 blue, entire. 



Gelatin stab: No liquefaction. 



Agar colonies: Small, circular, yellow- 

 ish or reddish-yellow, entire, becoming 

 lobed, grajash-green, iridescent. The 

 medium becomes dirty grayish-green. 



Agar slant: Smooth, spreading, slimy, 

 glistening, grayish-green to dark green, 

 fluorescent. 



Broth: Turbid green, iridescent to 

 opalescent with slimy sediment. 



Litmus milk: Not coagulated, blue 

 ring. 



Potato: Slimy, glistening, spreading, 

 steel blue. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 25''C. 



Source: Isolated from air. 



2. Pseudomonas putida (Trevisan) 

 Migula. {Bacillus Jluorcscens putidus 

 Flugge, Die Mila-oorganismen, 2 Aufl., 

 1886, 288; Bacillus putidus Trevisan, 

 I gen. e le specie d. Batteriacee, 1889, 18; 

 Migula, in Engler and Prantl, Die na- 

 tiir. Pflanzenfam., 1, la, 1895, 29; Ba- 

 cillus fluorescens putridus (sic) Kruse, 

 in Flugge, Die Mikroorganismen, 2, 

 1896, 292; Bacterium putidum Lehmann 

 and Neumann, Bakt. Diag., 1 Aufl., 2, 

 1896, 271 ; Pseudomonas putrida (sic) 

 Migula, Syst. d. Bakt., 2, 1900, 912.) 

 It is not clear which spelling should be 

 used. Either is correct. From Latin 

 putida or putrida, rotten, stinking. 

 Rods, with rounded ends. Motile, 



