FAMILY PSEUDOMONADACEAE 



97 



possessing polar flagella. Gram-negative. 



Gelatin colonies : Small, finely granular, 

 fluorescent with dark center, surrounded 

 by a yellow zone, with pale gray margin. 



Gelatin stab : Dirty-white surface 

 growth, becoming greenish, fluorescent. 

 No liquefaction. 



Agar colonies : Circular, raised, smooth, 

 amorphous, entire, with fluorescent zone 

 around the periphery. 



Agar slant : Yellowish-green layer, be- 

 coming fluorescent. 



Broth: Turbid, fluorescent. 



Litmus milk: Unchanged. 



Potato: Thin, gray to brownish, slimj^ 

 layer. 



Cultures give off odor of trimethyl- 

 amine. 



Indole not formed. 



Nitrites are produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 25°C. Will grow 

 at 37°C. (Pteid et al., Penn. Agr. Exp. 

 Sta., Bull. 422, 1942, 9). 



Distinctive features: Identical with 

 Pseudomonas fluorescens Migula accord- 

 ing to Lehmann and Neumann {loc. cit.) 

 except that it does not liquefy gelatin. 

 See Pseudomonas eisenbergii Migula. 



Habitat; Putrefying materials; water. 



23. Pseudomonas scissa (Frankland 

 and Frankland) Migula. {Bacillus scis- 

 8MS G. and P. Frankland, Ztschr. f. Hyg., 

 6, 1889, 398; Migula, Syst. d. Bakt., 2, 

 1900, 927.) From Latin, scissus, p.p. of 

 scindo, to cut. 



Rods: 0.5 by 0.5 to 1.0 micron, with 

 rounded ends, occurring singly, in pairs 

 and in chains; on gelatin, coccus-like. 

 Motile with presumably polar flagella. 

 Gram-negative. 



Gelatin colonies: Small, greenish. 



Gelatin stab : Thin, smooth, ghstening 

 surface growth, irregular, serrate margin. 

 No liquefaction. The medium becomes 

 light green in color. 



Agar slant: Smooth, glistening, lobed. 

 The medium assumes a greenish color. 



Broth: Turbid, with whitish sediment. 



Litmus milk : Not coagulated. 



Potato : Glistening, reddish-brown 

 growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Distinctive characters : Resembles 

 Pseudomonas viscosa Migula. 



Source : Found in water and soils in 

 Kent, England. 



Habitat : Water and soil. 



24. Pseudomonas ovalis Chester. 

 (Bacillus fluorescens ovalis Ravenel, 

 Memoirs Nat. Acad. Sci., 8, 1896, 9; 

 Chester, Determinative Bacteriologj^, 

 1901, 325; not Bacillus ovalis Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 435.) 

 From ovum, egg; M. L. oval. 



Rods: 0.3 to 0.7 by 0.7 to 1.3 microns, 

 occurring singly. Motile, possessing a 

 single polar flagellum. Gram-negative. 



Gelatin colonies : Irregular, lobate, 

 slightly granular. 



Gelatin stab : No liquefaction. 



Agar colonies : Circular, opaque, entire, 

 ureenish fluorescence. 



Agar slant: Thick, white, becoming 

 greenish^ fluorescent. 



Broth: Turbid, with pellicle. 



Litmus milk : No coagulation ; alkaline. 



Potato: Luxuriant, dirty-brown. 



Indole not formed. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



Blood serum not liquefied. 



Acid from glucose. 



Aerobic, facultative. 



Optimum temperature 25°C. 



Habitat: Soil. Has been found in in- 

 testinal canal. 



25. Pseudomonas striata Chester. 

 (Bacillus striatus viridis Ravenel, 

 Memoirs Nat. Acad. Sci., 8, 1896, 22; 

 Chester, Determinative Bacteriology, 

 1901, 325.) From Latin, strio, streak, 

 groove. 



Slender rods, of variable lengths, stain- 



