MANUAL OF DETERMINATIVE BACTERIOLOGY 



ing irregularly, occurring singly and in 

 pairs. Motile, possessing polar flagella. 

 Gram -negative. 



Gelatin colonies: Circular, yellowish, 

 with filamentous border. 



Gelatin stab: Raised, white surface 

 growth. No liquefaction. 



Agar slant: Thin, yellowish-green, 

 smooth, glistening. 



Broth: Turbid, becoming slightly 

 greenish. 



Litmus milk : No coagulation ; becom- 

 ing alkaline; litmus reduced. 



Potato : Moist, glistening, becoming 

 chocolate-brown. 



Indole is formed. 



Nitrites are produced from nitrates. 



Aerobic. 



Optimum temperature 25°C. 



Habitat: Soil. 



26. Pseudomonas denitrificans Ber- 

 gey et al. {Bacillus denitrificans fluo- 

 rescens Christensen, Cent. f. Bakt., II 

 Abt., 11, 1903, 190; Bergey etal.. Manual, 

 1st ed., 1923, 131.) From Latin, de, 

 from, out of; nitrum, soda, nitre; M. L. 

 denitrifying. 



Rods: 0.5 to 0.7 by 0.5 to 1.25 microns, 

 occurring singly and in pairs in large, 

 slimy masses. Motile. Gram-negative. 



Gelatin colonies: Small, circular, con- 

 toured, raised, moist, pearly-gray, glis- 

 tening. 



Gelatin stab: Whitish, lobed surface 

 growth. Yellowish-green growth in stab. 

 No liquefaction. 



Agar colonies : Pearly white, circular, 

 entire. 



Agar slant: Broad, whitish, contoured, 

 moist, entire. 



Broth: Turbid, with thick, wrinkled 

 pellicle. 



Litmus milk : Not coagulated. 



Potato: Reddish-gray layer. 



Indole not formed. 



Nitrates reduced with production of 

 nitrogen. 



Aerobic, facultative. 



Optimum temperature 25°C. 



Habitat: Soil. 



27. Pseudomonas solaniolens Paine. 

 (Rept. Int. Conf. Phytopath. and Econ. 

 Ent. Holland, 1923, 77; Phytomonas so- 

 laniolens Bergey et al.. Manual, 3rd ed., 

 1930, 274.) From M. L. Solanum, a 

 generic name. 



Small oval rods: Motile with a polar 

 flagellum. Gram-negative. 



Produces an iridescence in gelatin. 



Gelatin: No liquefaction. 



Gelatin colonies: Round. Iridescence 

 in medium. 



Agar colonies : Pale buff. 



Litmus milk: Curd, with no sign of 

 digestion. 



Potato : Pale buff-colored growth, no 

 change in medium. 



Nitrites not produced from nitrates. 



Acid but not gas from glucose. No 

 acid or gas from lactose, sucrose, manni- 

 tol or glycerol. 



Starch: Action feeble. 



Optimum temperature 20° to 30°C. 



Aerobic, facultative. 



Source : Isolated from potato showing 

 internal rust spots. 



28. Pseudomonas eisenbergii Migula. 

 (Bacillus fluurescens non liquefaciens 

 Eisenberg, Bakt. Diag., 3 Aufl., 1891, 

 145; Bacillus fluorescens immobilis Kruse, 

 in Fliigge, Die Mikroorganismen, 2, 1896, 

 294; Migula, Syst. d. Bakt., 2, 1900, 913; 

 Bacterium immobile Chester, Man. De- 

 term. Bact., 1901, 180; Pseudomonas non- 

 liquefaciens Bergey et al., Manual, 1st 

 ed., 1923, 132.) Named for Eisenberg, 

 the bacteriologist who first reported this 

 species. 



Short, slender rods, with rounded ends, 

 occurring singly. Non-motile. Kruse 

 (loc. cit., p. 293) lists the motile form of 

 this organism as Bacillus fluorescens non 

 liquefaciens. Gram-negative. 



Gelatin colonies : Fern-like surface 

 colonies. Medium around colonies has a 

 pearly luster. 



Gelatin stab : Surface growth has fluo- 

 rescent shimmer. No liquefaction. 



Agar slant : Greenish layer. 



Broth: Turbid, fluorescent. 



