FAMILY P8EUDOMONADACEAE 



101 



fragaroidea came from butter; it liquefies 

 gelatin, coagulates milk, and grows at 



•33. Pseudomonas nebulosa (Wright) 

 Chester. {Bacillus nebulosus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 465; 

 Chester, Man. Determ. Bact., 1901, 311; 

 Achromobacter nehulosum Bergey et al., 

 Manual, 1st ed., 1923, 145; not Bacillus 

 nebulosus Halle, These de Paris, 1898; 

 not Bacillus nebulosus Vincent, Ann. 

 Inst. Past., 21, 1907, 69; not Bacillus 

 nebulosus Migula, Syst. d. Bakt., 2, 

 1900, 844; not Bacillus nebulosus Gores- 

 line, Jour. Bact., 27, 1934, 52.) From 

 Latin, nebula, mist. 



Medium-sized rods, occurring singly. 

 Motile, possessing polar flagella. Gram- 

 negative. 



Gelatin colonies: Thin, circular, gray, 

 translucent, hazy, with white center. 



Gelatin stab: Crateriform liquefaction. 



Agar slant: Thin, transparent streak. 



Broth: Turbid, with graj' sediment. 



Litmus milk: Alkaline; reduction of 

 litmus. 



Potato: Scanty growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Sugar gelatin in deep stab : Fair growth, 

 with some gas formation. 



Aerobic, facultative. 



Optimum temperature 30° to 35°C. 



Habitat : Water. 



Probable synonym : Pseudomonas cen- 

 Irifugans Chester. (Man. Determ. 

 Bact., 1901, 312; Bacillus centrifugans 

 Wright, Mem. Nat. Acad. Sci., 7, 1895, 

 462.) 



34. Pseudomonas coadunata (Wright) 

 Chester. (Bacillus coadunatus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 460; 

 Chester, iVIan. Determ. Bact., 1901, 310; 

 Achromobacter coadunatum, Bergey et al., 

 ^Lanual, 1923, 147.) 1st ed.. From Latin, 

 coadunatus, to unite closely. 



Medium-sized rods, with rounded 

 ends, occurring singly, in pairs and in 

 chains. Motile, possessing a polar flagel- 

 lum. Gram -negative. 



Gelatin colonies: Circular, brownish, 

 dense. 



Gelatin stab : Crateriform to stratiform 

 liquefaction. 



Agar slant : Gray, translucent, 

 spreading. 



Broth: Turbid, with gray pellicle and 

 sediment. The medium has a slight 

 greenish tint. 



Litmus milk: Acid; coagulated. 



Indole is formed. 



Nitrites not produced from nitrates. 



Sugar gelatin in deep stab : Good growth 

 of discreet and confluent whitish colonies. 

 Marked gas production; no liquefaction. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Habitat : Water. 



35. Pseudomonas multistriata (Wright) 

 Chester. {Bacillus multistriaius\N right. 

 Memoirs Nat. Acad. Sci., 7, 1895, 462; 

 Chester, Man. Determ. Bact., 1901, 310; 

 Achromobacter multistrialum Bergey et 

 al.. Manual, 1st ed., 1923, 147.) From 

 Latin, multus, many, much; striatus, 

 grooved. 



Medium-sized rods, with rounded ends, 

 occurring singly and in pairs. Motile, 

 possessing polar flagella. Gram-negative. 



Gelatin colonies : Circular, grayish- 

 white, translucent. 



Gelatin stab : Crateriform liquefaction. 



Agar slant : Narrow, translucent, gray- 

 ish streak. 



Broth: Turbid. 



Litmus milk : Slightly acid; coagulated. 



Potato : Grayish to creamy, thick, glis- 

 tening, viscid, spreading. 



Indole not formed. 



Nitrites not produced from nitrates. 



Sugar gelatin in deep stab : Vigorous 

 growth with marked gas production ; also 

 liquefaction. 



* Prof. E. R. Hitchner, Univ. of Maine, Orono, Maine assisted in rearranging the 

 descriptions of the acid and gas producing pseudomouads {Aeromonas) , April, 1943. 



