t'AMILY PSEtTDOMONADACEAE 



103 



mice, guinea pigs and rabbits, causing 

 hemorrhagic septicemia. 



Distinctive characters: Much like 

 Pseudo/nonas punctata (Guthrie and 

 Hitchner, Jour. Bact., 43, 1943, 52). 



Source : Isolated from frogs dead of 

 septicemia (red leg). 



Habitat : Water and infected fresh 

 water animals. 



38. Pseudomonas ichthyosmia (Ham- 

 mer) comb. nov. (Bacillus ichthyosmius 

 Hammer, Iowa Agr. Sta. Res. Bui. 38, 

 1917; Escherichia ichthyosmia Bergey et 

 al., Manual, 1st ed., 1923, 201; Proteus 

 ichthyosmius Bergey et al.. Manual, 4th 

 ed., 1934, 364.) From Greek, ichthys, a 

 fish; osme, an odor. 



Rods: 0.6 to 0.8 by 1.0 to M microns, 

 occur singh'. Motile with a single polar 

 flageilum (Breed). Gram-negative. 



Gelatin stab: Liquefaction. 



Agar colonies: Small, white, becoming 

 darker with age. 



Agar slant : Dirty white, viscid growth. 



Broth : Turbid with gray sediment. 



Litmus milk: Acid. Litmus reduced. 

 Cultures have fishy odor. 



Potato : Thin, glistening layer. 



Indole is formed. 



Nitrites produced from nitrates. 



Acid and gas from glucose, fructose, 

 galactose, maltose, sucrose, glycerol, 

 salicin and mannitol. Lactose, dulcitol, 

 rafhnose and inulin not fermented. 



Aerobic, facultative. 



Optimum temperature 20^C. 



Source : Isolated from can of evaporated 

 milk having a fishy odor. 



Habitat: Not known. 



39. Pseudomonas ambigua (Wright) 

 Chester. (Bacillus ambiguus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 439; 

 Chester, Man. Determ. Bact., 1901, 308; 

 Achromobacler ambiguum Bergey et al.. 

 Manual, 1st ed., 1923, 148.) From Latin, 

 ambiguus, doubtful, uncertain. 



Small rods, with rounded ends, occur- 

 ring singly, in pairs and in chains. Mo- 



tile, possessing a polar flageilum. Gram- 

 negative. 



Gelatin colonies : Gray, translucent, 

 slighth' raised, irregular, radiate, with 

 transparent margin. 



Gelatin stab: No liquefaction. 



Agar slant : Gray, limited, entire. 



Broth: Turbid, with gray sediment. 



Litmus milk: Acid, slowly coagulated. 



Potato : Gray to creamy, viscid, 

 spreading. 



Indole is formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 30° to 35°C. 



Habitat: Water. 



40. Pseudomonas sinuosa (Wright) 

 Chester. (Bacillus sinuosus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 440; 

 Chester, Man. Determ. Bact., 1901, 307; 

 Achromobacler sinosum (sic) Bergey et 

 al.. Manual, 1st ed., 1923, 148.) From 

 Latin, sinuosus, full of bends, sinuous. 



Medium-sized rods, with rounded ends, 

 occurring singly, in pairs and in chains, 

 ^lotile, possessing two to four polar 

 flagella. Gram-negative. 



Gelatin colonies : Thin, translucent, 

 irregular, center brownish. 



Gelatin stab: Grayish-white, glisten- 

 ing, translucent. No liquefaction. 



Agar slant : Scanty, grayish growth. 



Broth : Turbid, with gray sediment. 



Litmus milk: Unchanged. 



Potato : Grayish-white, moist, spread- 

 ing. 



Indole is formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 30° to 35°C. 



Habitat : Water. 



41. Pseudomonas cruciviae Gray^ and 

 Thornton. (Gray and Thornton, Cent, 

 f. Bakt., II Abt., 73, 1928, 91; Achro- 

 mobacter cruciviae Bergey et al.. Manual, 

 3rd ed., 1930, 218.) From Latin, crux, 

 a cross, via, way, road; from Waycross, 

 a place name. 



