104 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Rods: 1.0 by 1.0 to 3.0 microns, occur- 

 ring singly and in pairs. Motile with one 

 to five polar flagella. Gram-negative. 



Gelatin colonies: Circular, white with 

 buff center, convex, smooth, undulate. 



Gelatin stab : No liquefaction. 



Agar colonies : Circular or amoeboid, 

 white to buff, flat to convex, smooth, 

 entire. 



Agar slant: Filiform, pale buff, raised, 

 smooth, undulate. 



Broth : Turbid. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



No acid in carbohydrate media. 



Attack phenol and m-cresol. 



Aerobic, facultative. 



Optimum temperature 30 to 35°C. 



Habitat : Soil. 



42. Pseudomonas rugosa (Wright) 

 Chester. {Bacillus rugosus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 438; 

 not Bacillus rugosus Henrici, Arb. Bakt. 

 Inst. Tech. Hochsch. Karlsruhe, 1, 1894, 

 28; not Bacillus rugosus Chester, Deter- 

 minative Bacteriology, 1901, 220; Ches- 

 ter, Determinative Bacteriology, 1901, 

 323.) From Latin, rugosus, wrinkled. 



Small rods, with rounded ends, occur- 

 ring singly, in pairs and in chains. Mo- 

 tile, possessing one to four polar flagella. 

 Gram-negative. 



Gelatin colonies : Grayish, translucent, 

 slightly raised, irregular, sinuous, radi- 

 ately erose to entire. 



Gelatin stab: Dense, grayish-green, 

 limited, wrinkled, reticulate surface 

 growth. No liquefaction. 



Agar slant: Grayish-white, limited, 

 slightly wrinkled, translucent. 



Broth: Turbid, with grajdsh pelUcle 

 and sediment. 



Litmus milk: Acid, coagulated. 



Potato: Moist, glistening, brown. 



Indole is formed. 



Nitrites not produced from nitrates. 



Aerobic. 



Optimum temperature 30°C. 



Habitat: Water. 



43. Pseudomonas desmolyticum Gray 

 and Thornton. (Gray and Thornton, 

 Cent. f. Bakt., II Abt., 7S, 1928, 90; 

 Achromobacter desmolyticum Bergey et 

 al.. Manual, 3rd ed., 1930, 217.) From 

 Greek desinos, bond, band; lytikos, able 

 to dissolve. 



Rods : 0.7 to 0.8 by 2.0 to 3.0 microns, 

 occurring singly and in pairs. Motile, 

 with one to five polar flagella. Gram- 

 negative. 



Gelatin colonies: Circular, gray to 

 buff, raised or umbonate. Smooth, glis- 

 tening, entire. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular or amoeboid, 

 whitish, flat or convex, smooth, translu- 

 cent to opaque, entire. 



Agar slant: Filiform, pale buff, raised, 

 smooth, undulate. 



Broth : Turbid. 



Nitrites produced from nitrates. 



Starch not hydrolyzed. 



Acid from glucose. 



Attack phenol and naphthalene. 



Aerobic, facultative. 



Optimum temperature 25°C. 



Habitat: Soil. 



44. Pseudomonas rathonis Gray and 

 Thornton. (Gray and Thornton, Cent, 

 f. Bakt., II Abt., 73, 1928, 90; Achromo- 

 bacter rathonis Bergey et al., Manual, 

 3rd ed., 1930, 216.) From M. L. of 

 Ratho Park (Edinburgh). 



Small rods: 0.5 to 1.0 by 1.0 to 3.0 

 microns. Motile, with polar flagella. 

 Gram-negative. 



Gelatin colonies: Circular, white, 

 raised, smooth, glistening, undulate. 



Gelatin stab: No Uquefaction. 



Agar colonies: Circular, buff, flat, 

 smooth, glistening, entire. 



Agar slant : Filiform, pale buff, convex, 

 smooth, glistening, undulate. 



Broth : Turbid, with peUicle. 



Nitrites produced from nitrates. 



Starch hydrolyzed. 



Acid from glucose and glycerol. 



