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MANUAL OF DETERMINATIVE BACTERIOLOGY 



Litmus milk : Acid, not digested. 

 Potato: No apparent growth. 

 Indole is formed. 

 Nitrites produced from nitrates. 

 Ammonia is produced. 

 Acid from glucose, lactose, maltose, 

 sucrose and starch. 

 Aerobic, facultative. 

 Optimum temperature 20°C. 

 Habitat : Soil. 



49. Pseudomonas tralucida Kellerman 

 et al. (Kellerman, McBeth Scales and 

 Smith, Cent. f. Bakt., II Abt., 39, 1913, 

 37; Cellulomonas tralucida Bergey et al., 

 Manual 1st ed., 1923, 163.) From Latin, 

 clear, transparent. 



Rods : 0.6 by 1.2 microns. Motile with 

 one or two polar flagella. Gram-negative. 



Gelatin stab : No liquefaction. 



Agar slant: Scant, grayish growth. 



Broth : Turbid. 



Litmus milk : Acid. 



Potato: No growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Ammonia not produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch, glycerol and mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Habitat: Soil. 



50. Pseudomonas mira McBeth. (Mc- 

 Beth, Soil Science, 1, 1916, 437; Cellu- 

 lonwnas mira Bergey et al.. Manual, 1st 

 ed., 1923, 165.) From Latin, minis, won- 

 derful, extraordinary. 



Rods : 0.4 by 1.6 microns. Motile with 

 a single polar flagellum. Gram-negative. 



Gelatin stab: Good growth. No lique- 

 faction. 



Agar colonies : Circular, convex, gray- 

 ish-white, granular, lacerate. 



Agar slant: Moderate, flat, grayish- 

 white, somewhat iridescent. 



Broth: Turbid. 



Litmus milk: Alkaline. 



Potato: Moderate, grayish-white. 



Indole not formed. 



Nitrites produced from nitrates. 

 Ammonia is produced. 

 Acid from glucose, maltose, lactose, 

 sucrose, starch, glycerol and mannitol. 

 Aerobic, facultative. 

 Optimum temperature 20°C. 

 Habitat: Soil. 



51. Pseudomonas lindneri Kluyver 

 and Hoppenbrouwers. (Lindner, 50 Ju- 

 bilaumsber. Westpreuss. Bot.-Zool. 

 Vereins, 1928, 253; T ermohacterium mo- 

 bile Lindner, Atlas d. Mikrosk. Grundl. 

 d. Garungsk., 3 Aufl., 2, 1928, Taf. 68; 

 Kluyver and Hoppenbrouwers, Arch. f. 

 Mikrobiol., 2, 1931, 259; Achromobacter 

 mobile Kluyver and Hoppenbrouwers, 

 ibid., 258; not Pseudomonas mobilis 

 Migula, Syst. d. Bakt., 2, 1900, 923.) 

 Named for Lindner, the German bacteri- 

 ologist who first studied this organism. 



Short rods 1.4 to 2.0 by 4.0 to 5.0 mi- 

 crons. Occurring singly, in pairs and 

 short chains. Motile with a single polar 

 flagellum. Gram-negative. 



Peptone gelatin: Poor growth. 



Peptone agar: Poor growth. 



Wort agar: White, round, raised colo- 

 nies, 1 mm. in diameter. Good growth. 

 Still better where 2 per cent sucrose, or 

 yeast extract with sucrose is added. 

 Chalk added to neutralize acid. 



Broth : Poor growth in peptone or yeast 

 extract broth unless sugars are added. 



Carbon dioxide, ethyl alcohol and some 

 lactic acid produced from glucose and 

 fructose, but not from mannose. May 

 or may not ferment sucrose. May pro- 

 duce as much as 10 per cent alcohol. 



Catalase produced. 



Anaerobic, facultative. 



Optimum temperature 30°C. 



Distinctive character: The fermenta- 

 tion resembles the alcoholic fermentation 

 produced by yeasts. 



Source : Isolated from the fermenting 

 sap (pulque) of Agave americana in 

 Mexico. 



Habitat: Fermenting plant juices in 

 tropical countries (Mexico). 



