108 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



No action on sugars. 

 Anaerobic, facultative. 

 Optimum temperature 20 to 25°C. 

 Habitat : Sea water of Norwegian coast. 



55. Pseudomonas calcis (Drew) 

 Kellerman and Smith. {Bacterium cal- 

 cis Drew, Yearbook Carnegie Inst. 

 Wash., 11, 1912, 136-144; Kellerman and 

 Smith, Proc. Nat. Acad. Sci., 4, 1914, 

 400.) From Latin calx (calc-), lime. 



Ovoid rods, 1.1 by 1.5 to 3 microns, 

 usually single but may form long chains. 

 Actively motile with one polar flagellum. 

 Gram-negative. 



Grows best in sea water or 3 per cent 

 salt media. Deposits CaCOs. 



Agar colonies : Circular, with finely 

 irregular outline, granular appearance, 

 elevated, spreading; old colonies having 

 brownish tinge in center. 



Gelatin stab : Infundibuliform lique- 

 faction. 



Gelatin colonies: Small, with lique- 

 faction. 



Broth : Good growth especially in pres- 

 ence of potassium nitrate, peptone or 

 calcium malate. 



Acid from glucose, mannite and sucrose 

 but not from lactose. 



Nitrates reduced to nitrites and am- 

 monia. 



Aerobic, facultative. 



Optimum temperature 20 to 28°C. 



Habitat : Sea water and marine mud. 



Bavendarmn (Arch. f. Mikrobiol., 3, 

 1932, 214) states that Pseudomonas calcis 

 is probably synonymous with Bacterium 

 brandti, Bacterium bauri and Bacterium 

 feiteli described by Parlandt (Bull. 

 Jard. imp. Bot. St. Petersburg, 11, 1911, 

 97-105). 



56. Pseudomonas calciprecipitans Mo- 

 lisch. (Cent. f. Bakt., II Abt., 65, 1925, 

 130.) From Latin, calx (calc-), lime; 

 praecipito, to cast down headlong, to 

 precipitate. 



Thin rods: 0.5 to 0.8 by 1.5 to 3.6 mi- 

 crons, with rounded ends, often staining 



irregularly. Motile, with one polar flagel- 

 lum. Gram -negative. 



Gelatin colonies : Circular, light brown 

 in color (large colonies show CaCOs 

 crystals). 



Gelatin stab : Surface growth with fili- 

 form growth in depth. Liquefaction 

 starts at bottom. 



Agar colonies (sea water). Grayish- 

 white, glistening. In two to three weeks 

 crystals of calcium carbonate form in the 

 agar. 



Agar slant: Slight, whitish, surface 

 growth, becoming thick, spreading, glis- 

 tening, with abundant CaCOs crystals in 

 medium. 



Ammonia formed. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Habitat : Sea water. 



57. Pseudomonas ichthyodermis (Wells 

 Zobell) Zobell and Upham. (Achromo- 

 bacter ichthyodermis Wells and Zobell, 

 Proc. Nat. Acad. Sci., 20, 123, 1934; 

 Zobell and Upham, Bull. Scripps Inst. 

 Oceanography, S, 1944, 246 and 253.) 

 From Greek, ichthys, a fish; derma, sldn. 



Small rods, 0.9 to 1.3 by 3 to 5 microns, 

 occurring singly and in pairs. No spores. 

 Encapsulated. Polar flagella. Pleomor- 

 phic forms predominate in old cultures. 

 Gram-negative. 



Requires sea water following initial 

 isolation. The following differential me- 

 dia are prepared with sea water. 



Agar colonies : Glistening, colorless, 

 convex, circular colonies 2 to 4 mm. in 

 diameter. 



Agar slants : Abundant, filiform, raised, 

 smooth, opalescent growth. 



Gelatin tube : Rapid crateriform lique- 

 faction complete in 5 days at 18°C. 



Sea water broth : Turbidity, with pelli- 

 cle, little granular sediment and no odor. 



Milk: No growth. Casein digested 

 when 3 per cent salt is added. 



Potato : No growth unless dialyzed in 

 sea water. Then fair growth with nc 

 pigment. 



