FAMILY PSEUDOMONADACEAE 



113 



referring to the type of lesion caused 

 on the blades of oats. 



Rods: 0.66 by 1.76 microns. Motile 

 with one to several flagella. Capsules. 

 Gram-negative. 



Green fluorescent pigment produced. 



Gelatin: Liquefied. 



Beef -peptone agar colonies : White, 

 raised, margins entire or slightly undu- 

 lating. 



Broth : Clouding in layers. Ring and 

 slight pellicle. 



Milk: Alkaline, sometimes a soft curd 

 which digests or clears. 



Slight production of nitrites from 

 nitrates. 



Indole not produced. 



Acid but not gas from glucose, fructose 

 and sucrose. No acid from lactose, mal- 

 tose, glycerol and mannitol. 



Starch: Hydrolysis slight. 



Optimum temperature 22°C. 



Optimum pH 6.5 to 7.0. 



Aerobic. 



Distinctive characters: Differs from 

 Pseudomonas coronafaciens in that the 

 cells are somewhat smaller and the patho- 

 gen produces a streak on oat blades 

 instead of a halo spot. 



Source : Forty cultures isolated from 

 oats gathered in various parts of America. 



Habitat : Pathogenic on cultivated oats, 

 and to a slight degree, on barley. 



67. Pseudomonas tomato (Okabe) 

 comb. 710V. {Bacterium tomato Okabe, 

 Jour. Soc. Trop. Agr. Formosa, 5, 1933, 32; 

 Phytomonas tomato Magrou, in Hauduroy 

 et al.. Diet. d. Bact. Path., Paris, 1937, 

 422.) Named for the host plant, tomato. 



Probable synonym : Bacterium punc- 

 tidans Bryan, Phytopath., 23, 1933, 897. 



Rods : 0.69 to 0.97 by 1.8 to 6.8 microns. 

 Motile with 1 to 3 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Slow liquefaction. 



Beef -extract agar colonies: White, 

 circular, flat and glistening. 



Broth: Turbid in 24 hours. Pellicle. 



Milk: Becomes alkaline and clears. 



Nitrites are usually produced from 

 nitrates. 



Indole not produced. 



No H2S produced. 



Acid but not gas from glucose, sucrose 

 and lactose. No acid from maltose and 

 glycerol. 



Starch hydrolysis feeble. 



Slight growth in 3 per cent salt. 



Optimum temperature 20° to 25°C. 

 Ma.ximum 33°C. 



Aerobic. 



Source : Isolated from diseased tomato 

 leaves. 



Habitat : Pathogenic on tomato, Lyco- 

 persicon esculentum. 



68. Pseudomonas aceris (Ark) Burk- 

 holder. {Phjitomonas aceris Ark, Phyto- 

 path., £9, 1939, 969; Burkholder, Phyto- 

 path., 32, 1942, 601.) From Latin acer, 

 maple; M.L. Acer, generic name. 



Rods: 0.3 to 0.8 by 0.8 to 2.5 microns. 

 Motile with 1 to 2 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced. 



Gelatin: Liquified. 



Beef -extract-peptone agar : Colonies are 

 grayish-white. Appearing in 24 hours. 



Broth: Turbid. 



Milk: Clearing with no coagulation. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid from glucose, fructose, galac- 

 tose, arabinose, xylose, sucrose, maltose, 

 lactose, raffinose, mannitol, glycerol and 

 dulcitol. 



Slight growth in broth plus 6 per cent 

 salt (Burkholder). 



Temperature : 13° to 3rC. 



Source : From diseased leaves of the 

 large leaf maple, Acer macrophillum . 



Habitat: Causes a disease of Acer spp. 



69. Pseudomonas angulata (Fromme 

 and Murray) Holland. (Bacterium an- 

 gulatum Fromme and Murray, Jour. Agr. 

 Res., 16, 1919, 219; Holland, Jour. Bact., 

 5, 1920, 224; Phytomonas angulata Bergey 



