114 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



et al., Manual, 3rd ed., 1930, 267.) From 

 L. angulatus, referring to the type of 

 lesion produced on the tobacco leaf. 



Description taken from Clara (Cornell 

 Agr. Exp. Sta. Mem. 159, 1934, 24). 



Rods : 0.75 to 1.5 by 1.5 to 3.0 microns. 

 Motile by 1 to 6 polar flagella. Gram- 

 negative. 



Gelatin: Liquefaction. 



Green fluorescent pigment produced. 



Beef -extract agar colonies : Dull white, 

 circular, raised, smooth and glistening. 



Broth: Turbid in 36 hours and 

 greenish. 



Milk: Alkaline. 



Nitrites not produced from nitrates. 



Indole not produced. 



No H2S produced. 



Lipolytic action negative (Starr and 

 Burkholder, Phytopath., 32, 1942, 601). 



Acid but not gas from glucose, galac- 

 tose, fructose, mannose, arabinose, xy- 

 lose, sucrose and mannitol. Alkaline reac- 

 tion from salts of citric, malic, succinic and 

 tartaric acid. Rhamnose, maltose, lac- 

 tose , raffinose , glycerol , sali ci n , and aceti c , 

 lactic and formic acids are not fermented. 



Starch not hydrolyzed. 



Slight growth in broth plus 5 to 6 per 

 cent salt (Burkholder). 



Facultative anaerobe. 



Distinctive characters: Braun (Phyto- 

 path., 27, 1937, 283) considers this species 

 to be identical in culture with Pseudo- 

 monas tabaci, but they differ in the type 

 of disease they produce. 



Sources: Isolated by Fromme and 

 Murray from small angular leaf spots on 

 tobacco. 



Habitat : Causes the angular leaf spot 

 of tobacco {Nicotiana tahacum). 



70. Pseudomonas aptata (Brown and 

 Jamieson) Stevens. {Bacterium aptatum 

 Brown and Jamieson, Jour. Agr. Res., 1, 

 1913, 206; Phytomonas aptata Bergey et 

 al., Manual, 1st ed., 1923, 184; Stevens, 

 Plant Disease Fungi, New York, 1925, 

 22.) From Latin aptatus adapted. 



Rods: 0.6 to 1.2 microns. Motile with 

 bipolar flagella. Gram -negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin : Liquefaction. 



Agar slants : Moderate growth along 

 streak, filiform, whitish, glistening. 



Broth : Turbid : A pellicle formed. 



Milk : Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole not produced in 10 days. Slight 

 amount found later. 



No HoS produced. 



Acid from glucose, galactose and 

 sucrose. No acid from lactose, maltose 

 and mannitol (Paine and Banfoot, Ann. 

 Appl. Biol., 11, 1924,312). 



Starch not hydrolyzed. 



Slight growth in broth plus 7 per cent 

 salt (Burkholder). 



Optimum temperature 27° to 28°C. 

 Maximum 34° to 35°C. Minimum below 

 1°C. 



Aerobic. 



Source : Isolated from diseased nas- 

 turtium leaves from Virginia and diseased 

 beet leaves from Utah. 



Habitat : Pathogenic on sugar beets, 

 nasturtiums, and lettuce. 



71. Pseudomonas primulae (Ark and 

 Gardner) Starr and Burkholder. (Phy- 

 tomonas primulae Ark and Gardner, 

 Phytopath., 26, 1936, 1053; Starr and 

 Burkholder, Phytopath., 82, 1942, 601.) 

 From L. primulus, first; M.L. Primula, 

 a generic name. 



Rods: 0.51 to 0.73 by 1.0 to 3.16 

 microns. Motile with a polar flagellum. 

 Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefaction. 



Agar colonies : Round, convex, smooth, 

 glistening, yellowish. 



Milk: Coagulated. 



Nitrites not produced from nitrates. 



Indole not produced. No H2S produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., S^, 1942,601). 



Acid but not gas from glucose, lactose, 

 sucrose, maltose, galactose, arabinose. 



