FAMILY PSEUDOMONADACEAE 



115 



glycerol, dulcitol and mannitol. Starch 

 not hydrolyzed. 



Growth in broth plus 5 per cent salt. 



Optimum temperature 19° to 22°C. 

 Maximum 34°C. Minimum 10°C. 



Optimum pH 6.8 to 7.0. Minimum 

 4.5 to 5.0. 



Facultative anaerobe. 



Source : Isolated from leaf-spot of 

 Primula polyantha. 



Habitat : Pathogenic on Primrd'a spp. 



72. Pseudomonas viridilivida (Brown) 

 Holland. (Bacterium viridilividum 

 Brown, Jour. Agr. Res., 4, 1915, 475; 

 Holland, Jour. Bact., 5, 1920, 225; Phylo- 

 monas viridilivida Bergey et al., ^Manual, 

 1st ed., 1923, 187.) From Latin, viridis, 

 green; lividus, blue. 



Rods : 1 .0 to 1 .25 by 1 .25 to 3.0 microns. 

 Motile with 1 to 3 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin : Slow liquefaction. 



Beef agar colonies: Cream white, 

 round, smooth, translucent, edges entire. 



Broth: Turbid, becomes lime green. 



Milk: Alkaline and clears. 



Nitrites not produced from nitrates. 



Indole is produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid from glucose and sucrose (Burk- 

 holder). 



Grows well in 4.5 per cent salt. Grows 

 in 7 per cent salt (Burkholder). 



Maximum temperature 34.5°C. Mini- 

 mum 1.5°C. 



Aerobic. 



Source : Isolated from diseased lettuce 

 from Louisiana. 



Habitat: Pathogenic on lettuce, Lac- 

 tuca saliva. 



73. Pseudomonas delphinii (Smith) 

 Stapp. (Bacillus delphini Smith, 

 Science, 19, 1904, 417; Bacteriujii del- 

 phinii Bryan, Jour. Agr. Res., 28, 1924, 

 261 ; Stapp, in Sorauer, Handbuch der 

 Pflanzenkrankheiten, 2, 5 Auf.. 1928. 106; 



Phytomonas delphinii Bergey et al., 

 Manual, 3rd ed., 1930, 261.) From 

 Latin, delphin, a dolphin; ^LL. Del- 

 phinium, a generic name. 



Rods: 0.6 to 0.8 by 1.5 to 2.0 microns. 

 Chains present. Motile with 1 to 6 polar 

 flagella. Capsules. Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquified. 



Beef agar slants : Growth thin, smooth, 

 shining, transparent, margins entire, 

 crystals. Agar becomes dark brown. 



Broth : Turbid in 24 hours with delicate 

 pellicle. 



Milk: Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole not produced. 



No H2S produced. 



Lipolytic action negative (Starr and 

 Burkholder, Phytopath., 32, 1942, 601). 



Acid from glucose, galactose and 

 fructose; slightly acid from sucrose. No 

 acid from lactose, maltose, glycerol and 

 mannitol. 



Starch : Hydrolysis feeble. 



Weak growth in broth plus 4 per cent 

 salt. 



Optimum pH 6.7 to 7.1. pH range 5.6 

 to 8.6. 



Optimum temperature 25°C. Maxi- 

 mum 30°C. Minimum 1°C. or less. 



Source: Isolated from black spot of 

 delphinium. 



Habitat: Pathogenic on delphinium 

 causing a black spot in the leaves. 



74. Pseudomonas berberidis (Thorn- 

 berry and Anderson) Stapp. (Phyto- 

 monas berberidis Thornberry and Ander- 

 son, Jour. Agr. Res., 43, 1931, 36; Stapp, 

 Bot. Rev., 1, 1935, 407; Bacterium ber- 

 beridis Burgwitz, Phytopathogenic Bac- 

 teria, Leningrad, 1935, 153.) From ]\I.L. 

 Berberis, barberry, generic name. 



Rods: 0.5 to 1.0 by 1.5 to 2.5 microns, 

 occurring singly or in pairs. Motile with 

 2 to 4 polar flagella. Capsules present. 



Gram-negative (Burkholder) ; not 

 Gram-positive as stated in original de- 

 scription. 



