116 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Green fluorescent pigment produced in 

 culture (Burkholder). 



Gelatin: Not liquefied. 



Glucose agar slants: Growth moder- 

 ate, filiform at first, later beaded, raised, 

 smooth, white. Butyrous in consistency. 



Milk: Becomes alkaline. No other 

 change. 



Nitrites not produced from nitrates. 



Indole not produced. 



No H2S produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., S^, 1942,601). 



Acid from glucose, galactose, and 

 sucrose. Maltose and rhamnose not 

 utilized (Burkholder). 



No gas from carbohydrates. 



Starch not hydrolyzed. 



Optimum temperature 18°C. Maxi- 

 mum 30°C . Minimum 7°C . 



Aerobic. 



Sources: Repeated isolations from 

 leaves and twigs of barberry. 



Habitat: Pathogenic on barberry, Ber- 

 beris thunbergerii and B. vulgaris. 



75. Pseudomonas coronafaciens (Elli- 

 ott) Stapp. {Bacterium coronafaciens 

 Elliott, Jour. Agr. Res., 19, 1920, 153; 

 Phytomonas coronafaciens Bergey et al.. 

 Manual, 1st ed., 1923, 180; Stapp, in 

 Sorauer, Handbuch der Pflanzenkrank- 

 heiten, 2, 5 Auf., 1928, 20.) From L. 

 corona, halo ;/aciens, producing, referring 

 to the lesions on oat blades. 



Probable synonyms: Elliott (Bact. 

 Plant Pathogens, 1930, 122) lists as 

 synonyms. Bacillus avenae (Russell, 

 Johns Hopkins Univ. Thesis, 1892) and 

 Bacillus avenae Manns and Pseudomonas 

 avenae Manns (Ohio Agr. Exp. Sta. Bui. 

 210, 1909, 133; Phytomonas avenae Ber- 

 gey et al., Manual, 3rd ed., 1930, 263). 



Rods: 0.65 by 2.3 microns, occurring 

 in chains. Motile with polar flagella. 

 Capsules. Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin : Slow liquefaction. 



Nutrient agar colonies: White, becom- 



ing irregularly circular, flat with raised 

 margins. 



Broth: Slight turbidity in 24 hours. 

 Heavy pellicle formed. 



Milk: Alkaline. A soft curd formed 

 followed by clearing. Curd sometimes 

 absent. 



Nitrites not produced from nitrates. 



Indole not formed. 



No HjS formed. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 3^, 1942, 601). 



Acid but no gas from glucose and su- 

 crose. Starch hydrolysis slight. 



Slight growth in broth plus 2 per cent 

 salt. 



Optimum temperature 24° to 25°C. 

 Maximum 3rC. Minimum 1°C. 



Source : Numerous isolations from 

 blighted blades of oats. 



Habitat : Causes a halo spot on oats 

 (Avena sativa). Artificial inoculations 

 show barley {Hordeum vulgar e), rye 

 {Secale cereale) and wheat (Triticum 

 aeslivum) to be susceptible. 



75a. Pseudomonas coronafaciens var. 

 atropurpiirea (Reddyand Godkin) Stapp. 

 (Bacterium coronafaciens var. atropur- 

 pureum Rcddy and Godkin, Phytopath., 

 13, 1923, 81 ; Stapp, in Sorauer, Handbuch 

 der Pflanzenkrankheiten, 2, 5 Auf., 1928, 

 39; Phytomonas coronafaciens var. atro- 

 purpurca Magrou, in Hauduroy et al., 

 Diet. d. Bact. Path., Paris, 1937, 371.) 

 From L. ater, black, dark; purpureus, 

 purple, referring to the color of the lesion 

 on brome grass. 



Distinctive characters : This variety 

 differs from Pseudomonas coronafaciens 

 in that it infects the brome-grass, Bromus 

 inermis, where it produces a water soaked 

 spot which is dark purple in color. 



Source : Numerous isolations from dis- 

 eased brome-grass. 



Habitat : Pathogenic on Bromus 

 inermis and Agropyron repens. Has been 

 artificially inoculated on oats, Avena 

 sativa. 



